MSMEG_3221 Data
Gene Information
| Gene | Locus | Rv | Start | Stop | Strand |
|---|---|---|---|---|---|
| MSMEG_3221 | trpA | Rv1613 | 3299174 | 3299962 | plus |
Protein Information
| Protein Name | Protein Product | Length (Amino Acids) |
|---|---|---|
| tryptophan synthase subunit alpha | YP_887535.1 | 262 |
Essentiality Data
| Original Call | New Call | Comments | NSW | - | TC trpA low hits. Trp region 217221 all low. |
|---|
Knockout Information
| Knockout Strain | Status | Plate | Well | MSMEG_3221::zeo | Confirmed | D11 | KO4 |
|---|
Dendra Information
| Dendra Plasmid Strain | Status | Plate | Well | pMSMEG_3221.dendra | Confirmed | MSR6 | E12 |
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CRISPRi Information
| CRISPRi Plasmid Name | sgRNA Sequence | pMSMEG_3221.sgRNA | gatataatctgggaATGCGGTCGAGGTCGTTCGCCgtttttgtactcga |
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Protein Localization Images
| Composite 1 | Composite 2 | Summary Plot |  |
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| Heatmap Thumbnail | Spherocylindrical Projection | Density Plot | Demograph |  |
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