MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_6143.sgRNA MSMEG_6143 - gatataatctgggaGTCCGCAATTGGGTTGGGGCCCGGgtttttgtactcga hypothetical protein MSMEG_6143 YP_890364.1
pMSMEG_6153.sgRNA MSMEG_6153 - gatataatctgggaGCCCGAGGACACCAGCAGGGCGTCgtttttgtactcga DNA polymerase III subunit delta\' YP_890373.1
pMSMEG_6154.sgRNA MSMEG_6154 - gatataatctgggaGGTGACGCTGATCAGCGTCCGgtttttgtactcga adenylate cyclase YP_890374.1
pMSMEG_6157.sgRNA MSMEG_6157 topA gatataatctgggaGACGCCGTGCTCACCGCCGAAgtttttgtactcga DNA topoisomerase I YP_890377.1
pMSMEG_6182.sgRNA MSMEG_6182 - gatataatctgggaAGGCCGAGATCACGCGCGACGTgtttttgtactcga hypothetical protein MSMEG_6182 YP_890402.1
pMSMEG_6184.sgRNA MSMEG_6184 - gatataatctgggaGTGTCGAGAAGTTCGGCCAGGAAgtttttgtactcga alpha/beta hydrolase YP_890403.1
pMSMEG_6186.sgRNA MSMEG_6186 - gatataatctgggaGCCGTCGTCACGCAACTCCAGgtttttgtactcga hypothetical protein MSMEG_6186 YP_890406.1
pMSMEG_6189.sgRNA MSMEG_6189 - gatataatctgggaGCCACGTGTCGAGAAGTTCGGCgtttttgtactcga Crp/Fnr family transcriptional regulator protein YP_890409.1
pMSMEG_6191.sgRNA MSMEG_6191 - gatataatctgggaGGTCGGCGCCGAGGTGAGCGCgtttttgtactcga translation initiation inhibitor YP_890411.1
pMSMEG_6195.sgRNA MSMEG_6195 - gatataatctgggaACGACGCGGTTCGACGTGTCGGgtttttgtactcga iontransporting ATPase YP_890414.1
pMSMEG_6256.sgRNA MSMEG_6256 asd gatataatctgggaGCCCTCGGAGCGCGGCGAGGCgtttttgtactcga aspartatesemialdehyde dehydrogenase YP_890475.1
pMSMEG_6257.sgRNA MSMEG_6257 - gatataatctgggaGCGTACTCGACGCAGCGCAGCAgtttttgtactcga aspartate kinase YP_890476.1
pMSMEG_6271.sgRNA MSMEG_6271 leuA gatataatctgggaGATGGCGTCCTGGTGGCTGCCgtttttgtactcga 2isopropylmalate synthase YP_890490.1
pMSMEG_6272.sgRNA MSMEG_6272 - gatataatctgggaACACTTCGTCACCGATCAAGGgtttttgtactcga NADglutamate dehydrogenase YP_890491.1
pMSMEG_6276.sgRNA MSMEG_6276 - gatataatctgggaGGCCGCGACCACCTGCACACCgtttttgtactcga mur ligase YP_890495.1
pMSMEG_6277.sgRNA MSMEG_6277 - gatataatctgggaGCCGAGCGGACGTGCGTCGGAACgtttttgtactcga cobyric acid synthase YP_890496.1
pMSMEG_6285.sgRNA MSMEG_6285 - gatataatctgggaGTCTTACCGCAGCCGCGCGGACCgtttttgtactcga DNA polymerase III subunits gamma and tau YP_890504.1
pMSMEG_6286.sgRNA MSMEG_6286 - gatataatctgggaGCGCACGCCGTCGGCGTCGCCgtttttgtactcga aspartate transaminase YP_890505.1
pMSMEG_6330.sgRNA MSMEG_6330 - gatataatctgggaGCCCGAGCGCGGGCATCGGCACGGgtttttgtactcga prephenate dehydrogenase YP_890546.1
pMSMEG_6363.sgRNA MSMEG_6363 - gatataatctgggaGGCATCGACCCGCGGAAAGCGgtttttgtactcga cysteine desulfurase YP_890577.1
pMSMEG_6365.sgRNA MSMEG_6365 - gatataatctgggaGTCGGCACCGCCCGCAGCACCGGgtttttgtactcga hypothetical protein MSMEG_6365 YP_890578.1
pMSMEG_6366.sgRNA MSMEG_6366 - gatataatctgggaGTCGCGCGTCTCGATGCGCGGTgtttttgtactcga Oantigen export system, ATPbinding protein YP_890579.1
pMSMEG_6367.sgRNA MSMEG_6367 - gatataatctgggaACCGGCGAGGTCGCGGCGTGACAgtttttgtactcga glycosyl transferase family protein YP_890580.1
pMSMEG_6369.sgRNA MSMEG_6369 - gatataatctgggaAGACGTGCACGCTCAACGGCGgtttttgtactcga Oantigen export system, permease YP_890581.1
pMSMEG_6382.sgRNA MSMEG_6382 - gatataatctgggaACCGTTGCCGCCGACGGTCGCgtttttgtactcga oxidoreductase, FADbinding YP_890595.1
pMSMEG_6385.sgRNA MSMEG_6385 - gatataatctgggaGCAGGGCCTCGCCCAGGCCCAGgtttttgtactcga short chain dehydrogenase YP_890598.1
pMSMEG_6386.sgRNA MSMEG_6386 - gatataatctgggaGCACGGCGAGCACCATCTGGCgtttttgtactcga membrane protein YP_890599.1
pMSMEG_6387.sgRNA MSMEG_6387 - gatataatctgggaGCCCTTGGAAGCCGTAGTACGGgtttttgtactcga arabinosyltransferase A YP_890600.1
pMSMEG_6388.sgRNA MSMEG_6388 - gatataatctgggaGTGGCGACCGCGAGCGGGGCGAgtttttgtactcga arabinosyltransferase B YP_890601.1
pMSMEG_6391.sgRNA MSMEG_6391 - gatataatctgggaACCAAAGGCAGGTTGTAGGAATCgtttttgtactcga propionylCoA carboxylase subunit beta YP_890604.1
pMSMEG_6392.sgRNA MSMEG_6392 - gatataatctgggaGCGGCCTGCCGCTTGCGCGAGGgtttttgtactcga polyketide synthase YP_890605.1
pMSMEG_6393.sgRNA MSMEG_6393 - gatataatctgggaGGTCACCCTTAAAGCCGCCGGTgtttttgtactcga longchainfattyacidCoA ligase YP_890606.1
pMSMEG_6394.sgRNA MSMEG_6394 - gatataatctgggaGGTCTTGGGCAGGTTGAAGACgtttttgtactcga hypothetical protein MSMEG_6394 YP_890607.1
pMSMEG_6398.sgRNA MSMEG_6398 - gatataatctgggaGCCGCACGAACTCGACCTTCCAgtttttgtactcga antigen 85A YP_890611.1
pMSMEG_6400.sgRNA MSMEG_6400 - gatataatctgggaGACGAACGCCAGCGCCCCCTCgtttttgtactcga transmembrane protein YP_890613.1
pMSMEG_6401.sgRNA MSMEG_6401 - gatataatctgggaACCGCCTGCGATCGCGCGGATgtttttgtactcga phosphoribose diphosphate:decaprenylphosphate phosphoribosyltransferase YP_890614.1
pMSMEG_6403.sgRNA MSMEG_6403 - gatataatctgggaGACTCGTCGGCTTCGAGTGCGgtttttgtactcga bifunctional udpgalactofuranosyl transferase glft YP_890616.1
pMSMEG_6404.sgRNA MSMEG_6404 glf gatataatctgggaACGCTCGGCGATCGTCAGACCgtttttgtactcga UDPgalactopyranose mutase YP_890617.1
pMSMEG_6413.sgRNA MSMEG_6413 serS gatataatctgggaAAGCAGCGCACCGGCACCGGTgtttttgtactcga seryltRNA synthetase YP_890626.1
pMSMEG_6418.sgRNA MSMEG_6418 - gatataatctgggaGGTCAGGTCGATGTCGCGGATgtttttgtactcga prephenate dehydratase YP_890631.1
pMSMEG_6427.sgRNA MSMEG_6427 - gatataatctgggaGTCATCCCAGTTGACGACGTTgtttttgtactcga [Mn] superoxide dismutase [Mycobacterium smegmatis str. MC2 155] YP_890640.1
pMSMEG_6458.sgRNA MSMEG_6458 gltD gatataatctgggaGTTCCCCAGCGGGCATCCGTTGTGgtttttgtactcga glutamate synthase subunit beta YP_890671.1
pMSMEG_6459.sgRNA MSMEG_6459 - gatataatctgggaGCTCCATGAGCGACGCGTGGTACTCgtttttgtactcga ferredoxindependent glutamate synthase 1 YP_890672.1
pMSMEG_6565.sgRNA MSMEG_6565 - gatataatctgggaAGATGTCGCGGTCATCGGTCGCgtttttgtactcga hypothetical protein MSMEG_6565 YP_890777.1
pMSMEG_6576.sgRNA MSMEG_6576 - gatataatctgggaGCCGCCCGAAGGGTTTCCGGACgtttttgtactcga pyridoxamine 5\'phosphate oxidase YP_890788.1
pMSMEG_6583.sgRNA MSMEG_6583 - gatataatctgggaACATGGCGGCCGGTGTCGATGCgtttttgtactcga antigen 85C YP_890794.1
pMSMEG_6596.sgRNA MSMEG_6596 - gatataatctgggaGCTCGGTGGTCTTGGCGAACTCGGgtttttgtactcga hypothetical protein MSMEG_6596 YP_890808.1
pMSMEG_6636.sgRNA MSMEG_6636 - gatataatctgggaGAACGCAAGGTTCTTCTCGTGgtttttgtactcga [Mn] superoxide dismutase [Mycobacterium smegmatis str. MC2 155] YP_890845.1
pMSMEG_6638.sgRNA MSMEG_6638 metE gatataatctgggaGCTCTGGGCGCGCCACGTCGCCGTgtttttgtactcga 5methyltetrahydropteroyltriglutamate/homocysteine Smethyltransferase YP_890847.1
pMSMEG_6649.sgRNA MSMEG_6649 - gatataatctgggaGCCGACGCAGCGAGCGCTCACgtttttgtactcga hypothetical protein MSMEG_6649 YP_890858.1
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