MSRdb: Mycobacterial Systems Resource



CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_5435.sgRNA MSMEG_5435 - gatataatctgggaACCGAGCCGGTGGTCACCCATgtttttgtactcga longchainfattyacidCoA ligase YP_889674.1
pMSMEG_5436.sgRNA MSMEG_5436 ispE gatataatctgggaACCGAGCCGGTGGTCACCCATgtttttgtactcga 4diphosphocytidyl2CmethylDerythritol kinase YP_889675.1
pMSMEG_5440.sgRNA MSMEG_5440 - gatataatctgggaACAAGACGGTAACGAAAAGGTgtttttgtactcga deoxyribonuclease YP_889679.1
pMSMEG_5441.sgRNA MSMEG_5441 metG gatataatctgggaGTCGGTGTAGGCCGACAGTCGgtttttgtactcga methionyltRNA synthetase YP_889680.1
pMSMEG_5446.sgRNA MSMEG_5446 pabB gatataatctgggaGGTGCGCGCCACCGCGTCGGCgtttttgtactcga aminodeoxychorismate synthase component I YP_889684.1
pMSMEG_5469.sgRNA MSMEG_5469 - gatataatctgggaATTCCTCGGCCGTGATGGCCAgtttttgtactcga GNAT family acetyltransferase YP_889707.1
pMSMEG_5471.sgRNA MSMEG_5471 galU gatataatctgggaGGGCACAGTCTTGGTTGCCGGgtttttgtactcga UTPglucose1phosphate uridylyltransferase YP_889709.1
pMSMEG_5487.sgRNA MSMEG_5487 - gatataatctgggaATCGCACGCGCCGCGTCGGAGCGgtttttgtactcga sensor histidine kinase YP_889725.1
pMSMEG_5515.sgRNA MSMEG_5515 purH gatataatctgggaACCTGCAGCCCGTCGGGGAACGGgtttttgtactcga bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase YP_889752.1
pMSMEG_5517.sgRNA MSMEG_5517 - gatataatctgggaACTTCTTCGTCACCGAACTCGTCGACgtttttgtactcga hypothetical protein MSMEG_5517 YP_889754.1
pMSMEG_5524.sgRNA MSMEG_5524 sucD gatataatctgggaGGCCTCGATGATGGCGTCCTTgtttttgtactcga succinylCoA synthetase subunit alpha YP_889761.1
pMSMEG_5525.sgRNA MSMEG_5525 sucC gatataatctgggaGGTGCGGTTGGCGCGATCGAGgtttttgtactcga succinylCoA synthetase subunit beta YP_889762.1
pMSMEG_5536.sgRNA MSMEG_5536 - gatataatctgggaATGAGGAGACATCGATGAGCGAgtttttgtactcga chorismate mutase YP_889773.1
pMSMEG_5589.sgRNA MSMEG_5589 - gatataatctgggaACTTTTCTGTTCGCTACACCGGgtttttgtactcga manganese transporter MntH YP_889824.1
pMSMEG_5662.sgRNA MSMEG_5662 prrA gatataatctgggaGCCGACCTGGATGGTCTCCGAgtttttgtactcga DNAbinding response regulator PrrA YP_889895.1
pMSMEG_5663.sgRNA MSMEG_5663 - gatataatctgggaGGTCCGCAGCGACGGGGTGCGgtttttgtactcga sensortype histidine kinase PrrB YP_889896.1
pMSMEG_5672.sgRNA MSMEG_5672 gltA gatataatctgggaGCGCGTTGCGCGGGAAACCGTCgtttttgtactcga type II citrate synthase YP_889905.1
pMSMEG_5684.sgRNA MSMEG_5684 - gatataatctgggaACCGCGGCGGCGTCGACGTCGTCgtttttgtactcga phosphoserine aminotransferase YP_889917.1
pMSMEG_5685.sgRNA MSMEG_5685 - gatataatctgggaACCGCGGCGGCGTCGACGTCGTCgtttttgtactcga DNAbinding protein YP_889918.1
pMSMEG_5688.sgRNA MSMEG_5688 - gatataatctgggaGGGACCTCGACGTCGATCTTCTgtttttgtactcga MarR family transcriptional regulator YP_889921.1
pMSMEG_5706.sgRNA MSMEG_5706 - gatataatctgggaACTGAGGGACAAAAACCCAGCgtttttgtactcga DNA or RNA helicase of superfamily protein II YP_889939.1
pMSMEG_5773.sgRNA MSMEG_5773 - gatataatctgggaGCCCTGCTGGTTCTGGCCGGGgtttttgtactcga fatty acid desaturase YP_890001.1
pMSMEG_5785.sgRNA MSMEG_5785 - gatataatctgggaGCCTGCGGGATGAACGGGAACCgtttttgtactcga hypothetical protein MSMEG_5785 YP_890013.1
pMSMEG_5796.sgRNA MSMEG_5796 - gatataatctgggaGCGTCACCGGCGAGAGCCGGACgtttttgtactcga glycine cleavage Tprotein (aminomethyl transferase) YP_890024.1
pMSMEG_5797.sgRNA MSMEG_5797 - gatataatctgggaGCGTCGTTGACACCGTCGGAGgtttttgtactcga hypothetical protein MSMEG_5797 YP_890025.1
pMSMEG_5798.sgRNA MSMEG_5798 purM gatataatctgggaGCCAGGCCACCACCGGTGACGTGgtttttgtactcga phosphoribosylaminoimidazole synthetase YP_890026.1
pMSMEG_5800.sgRNA MSMEG_5800 purF gatataatctgggaGCCGGGGTGGCGAAATCGATGCCgtttttgtactcga amidophosphoribosyltransferase YP_890028.1
pMSMEG_5824.sgRNA MSMEG_5824 purL gatataatctgggaATCGGGGTGCTGCCGGTCTGGTTgtttttgtactcga phosphoribosylformylglycinamidine synthase II YP_890052.1
pMSMEG_5841.sgRNA MSMEG_5841 purC gatataatctgggaACCCGCCGTTCGCAGCAGGTGGTCgtttttgtactcga phosphoribosylaminoimidazolesuccinocarboxamide synthase YP_890068.1
pMSMEG_5847.sgRNA MSMEG_5847 purB gatataatctgggaATCTCGTCGCTTGCGTAGCGGTTGGgtttttgtactcga adenylosuccinate lyase YP_890074.1
pMSMEG_5852.sgRNA MSMEG_5852 purD gatataatctgggaACCACCGTCTCACCGTCGACCACgtttttgtactcga phosphoribosylamineglycine ligase YP_890079.1
pMSMEG_5892.sgRNA MSMEG_5892 - gatataatctgggaACCGGCGGGAAGGGGATGTGCAGgtttttgtactcga alpha,alphatrehalosephosphate synthase YP_890117.1
pMSMEG_5983.sgRNA MSMEG_5983 rfbA gatataatctgggaGATCTCGATCACGTCGTTGTCgtttttgtactcga glucose1phosphate thymidylyltransferase YP_890209.1
pMSMEG_6073.sgRNA MSMEG_6073 - gatataatctgggaGTCCAGATCGGTGCGGGGCACCTgtttttgtactcga TrmH family RNA methyltransferase YP_890295.1
pMSMEG_6074.sgRNA MSMEG_6074 cysS gatataatctgggaGCCCAGCGCGTCGTAGGCGGCgtttttgtactcga cysteinyltRNA synthetase YP_890296.1
pMSMEG_6075.sgRNA MSMEG_6075 ispF gatataatctgggaGTCGGTGCCGAGCCCGACGCGAGgtttttgtactcga 2CmethylDerythritol 2,4cyclodiphosphate synthase YP_890297.1
pMSMEG_6076.sgRNA MSMEG_6076 ispD gatataatctgggaGAAGCCCCGACAGAGCGTGCTgtttttgtactcga 2CmethylDerythritol 4phosphate cytidylyltransferase YP_890298.1
pMSMEG_6077.sgRNA MSMEG_6077 - gatataatctgggaGGTGTGCGGCGCGCGCAGCACgtttttgtactcga CarD family transcriptional regulator YP_890299.1
pMSMEG_6082.sgRNA MSMEG_6082 - gatataatctgggaGAACTCAAATCCTGTGGCAGCgtttttgtactcga carbonic anhydrase YP_890304.1
pMSMEG_6091.sgRNA MSMEG_6091 - gatataatctgggaATCGACAGACGCCGGAATCTGGgtttttgtactcga negative regulator of genetic competence ClpC/mecB YP_890312.1
pMSMEG_6092.sgRNA MSMEG_6092 - gatataatctgggaGCGCAACTTCGCGGCGTTTTTTgtttttgtactcga Lsr2 protein YP_890314.1
pMSMEG_6097.sgRNA MSMEG_6097 panC gatataatctgggaACGCGCGGTCGGGGCGAACGATgtttttgtactcga pantoatebetaalanine ligase YP_890318.1
pMSMEG_6098.sgRNA MSMEG_6098 - gatataatctgggaGCGGTGATGCCGAAGCACGTGTgtttttgtactcga chalcone/stilbene synthase YP_890319.1
pMSMEG_6101.sgRNA MSMEG_6101 folK gatataatctgggaGCCCGTCGTTGATCTTGGCGCGGACgtttttgtactcga 2amino4hydroxy6hydroxymethyldihydropteridine pyrophosphokinase YP_890322.1
pMSMEG_6103.sgRNA MSMEG_6103 folP gatataatctgggaGTCGATGAATTTCCCACCGTCgtttttgtactcga dihydropteroate synthase YP_890324.1
pMSMEG_6104.sgRNA MSMEG_6104 folE gatataatctgggaGTAGCCGACGTGCGCCACGCCgtttttgtactcga GTP cyclohydrolase I YP_890325.1
pMSMEG_6110.sgRNA MSMEG_6110 hpt gatataatctgggaGCGCCCTTGAGCACCGTGACCAgtttttgtactcga hypoxanthine phosphoribosyltransferase YP_890331.1
pMSMEG_6111.sgRNA MSMEG_6111 tilS gatataatctgggaGGGTCACGGTGCGCCGGATGCgtttttgtactcga tRNA(Ile)lysidine synthetase YP_890332.1
pMSMEG_6114.sgRNA MSMEG_6114 ppa gatataatctgggaGTCCTCGCCCAGGGTGTTCTCgtttttgtactcga inorganic pyrophosphatase YP_890335.1
pMSMEG_6142.sgRNA MSMEG_6142 - gatataatctgggaGCTTCACCGAGATCTGCGCGGCGAGgtttttgtactcga nucleosidediphosphatesugar epimerase YP_890363.1
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