MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_0880.sgRNA MSMEG_0880 groEL gatataatctgggaGTCAAGGACCTGCTGCCGCTGCTgtttttgtactcga molecular chaperone GroEL YP_885283.1
pMSMEG_0903.sgRNA MSMEG_0903 lpdA gatataatctgggaGTCTCCAAGGAGATCGAGAAGCAGTAgtttttgtactcga dihydrolipoamide dehydrogenase YP_885306.1
pMSMEG_0909.sgRNA MSMEG_0909 - gatataatctgggaAGACGAGAGCATGACGGGCACgtttttgtactcga acylACP thioesterase YP_885312.1
pMSMEG_0922.sgRNA MSMEG_0922 deoC gatataatctgggaAGACACGCGACAGGCCCCGCCgtttttgtactcga deoxyribosephosphate aldolase YP_885325.1
pMSMEG_0928.sgRNA MSMEG_0928 murB gatataatctgggaGTACCGACAGCACGCACCCCAgtttttgtactcga UDPNacetylenolpyruvoylglucosamine reductase YP_885331.1
pMSMEG_0934.sgRNA MSMEG_0934 - gatataatctgggaGCGGTGTTCGGGCTTCCCACCGAgtttttgtactcga hypothetical protein MSMEG_0934 YP_885337.1
pMSMEG_0935.sgRNA MSMEG_0935 - gatataatctgggaAACTCCAGCAACGTGTTGAAGgtttttgtactcga 2,3bisphosphoglycerateindependent phosphoglycerate mutase YP_885338.1
pMSMEG_0937.sgRNA MSMEG_0937 regX3 gatataatctgggaGTGGGATTGCGCCCACGTGCACAgtttttgtactcga DNAbinding response regulator RegX3 YP_885340.1
pMSMEG_0943.sgRNA MSMEG_0943 proC gatataatctgggaGACCATCGCCTCGGCCATCAGgtttttgtactcga pyrroline5carboxylate reductase YP_885346.1
pMSMEG_0948.sgRNA MSMEG_0948 - gatataatctgggaACCTCGAGCGTGTGCTGCACCGGGAgtttttgtactcga hypothetical protein MSMEG_0948 YP_885351.1
pMSMEG_0949.sgRNA MSMEG_0949 - gatataatctgggaACCCCGACTATTTCGAGGTCGGACGgtttttgtactcga HADsuperfamily hydrolase YP_885352.1
pMSMEG_0952.sgRNA MSMEG_0952 hemA gatataatctgggaGCCCGATCACCGACAGACCGCCgtttttgtactcga glutamyltRNA reductase YP_885355.1
pMSMEG_0953.sgRNA MSMEG_0953 hemC gatataatctgggaACGACGCGGTATCGCGGGGATgtttttgtactcga porphobilinogen deaminase YP_885356.1
pMSMEG_0954.sgRNA MSMEG_0954 hemD gatataatctgggaGCCCCACGCAGGCGATCTTCACGCCgtttttgtactcga uroporphyrinogenIII synthase YP_885357.1
pMSMEG_0956.sgRNA MSMEG_0956 hemB gatataatctgggaGTCGATGCCGTCGGCGACGAACAgtttttgtactcga deltaaminolevulinic acid dehydratase YP_885358.1
pMSMEG_0969.sgRNA MSMEG_0969 hemL gatataatctgggaGCTGTGGCCGTGGTAGCAGCCgtttttgtactcga glutamate1semialdehyde aminotransferase YP_885371.1
pMSMEG_0970.sgRNA MSMEG_0970 - gatataatctgggaAGTCGTGGAACACCTTCGAGGGgtttttgtactcga phosphoglycerate mutase YP_885372.1
pMSMEG_0971.sgRNA MSMEG_0971 - gatataatctgggaGCCGCGGCTTTGGGGCGGATCgtttttgtactcga hypothetical protein MSMEG_0971 YP_885373.1
pMSMEG_0972.sgRNA MSMEG_0972 - gatataatctgggaACGACACCGCCGATGCGTTGCAgtttttgtactcga cytochrome C biogenesis protein transmembrane region YP_885374.1
pMSMEG_0973.sgRNA MSMEG_0973 - gatataatctgggaGAACGCCGCGGGTGAGGCCGTgtttttgtactcga hypothetical protein MSMEG_0973 YP_885375.1
pMSMEG_0974.sgRNA MSMEG_0974 ccsB gatataatctgggaGACCGCGGCGGCCACCAGCCCgtttttgtactcga cytochrome C biogenesis protein CcsB YP_885376.1
pMSMEG_0977.sgRNA MSMEG_0977 - gatataatctgggaACGAGCAGGCCCAGCAGCGAGAAgtttttgtactcga hypothetical protein MSMEG_0977 YP_885379.1
pMSMEG_0988.sgRNA MSMEG_0988 menA gatataatctgggaGCCGAGCACCGCGACCAGACCgtttttgtactcga 1,4dihydroxy2naphthoate octaprenyltransferase YP_885390.1
pMSMEG_1011.sgRNA MSMEG_1011 - gatataatctgggaACCTCATGGGGCCCATCCACGTCATgtttttgtactcga short chain dehydrogenase YP_885412.1
pMSMEG_1017.sgRNA MSMEG_1017 - gatataatctgggaGAGTGCCTTGATGCGGTCGGGgtttttgtactcga glutaredoxin YP_885418.1
pMSMEG_1018.sgRNA MSMEG_1018 - gatataatctgggaGTCTCGGAAAACACGCATCGTTTCGTgtttttgtactcga Pseudogene
pMSMEG_1019.sgRNA MSMEG_1019 - gatataatctgggaGGCACCCAGCTGATTGGCGTAgtttttgtactcga ribonucleotidediphosphate reductase subunit alpha YP_885419.1
pMSMEG_1033.sgRNA MSMEG_1033 nrdF2 gatataatctgggaATCTCGGCGGTCGAGCACAGCGTgtttttgtactcga ribonucleotidediphosphate reductase subunit beta YP_885432.1
pMSMEG_1037.sgRNA MSMEG_1037 - gatataatctgggaATCTCCGGCCGGACGTCGTGTTCGGCgtttttgtactcga alcohol dehydrogenase YP_885435.1
pMSMEG_1040.sgRNA MSMEG_1040 - gatataatctgggaGCGCCATGAGGCCGCCGACCAGgtttttgtactcga Pseudogene
pMSMEG_1041.sgRNA MSMEG_1041 serB gatataatctgggaGCCACGTCGGACGGCGCGGCCAgtttttgtactcga phosphoserine phosphatase YP_885438.1
pMSMEG_1046.sgRNA MSMEG_1046 - gatataatctgggaACGATGAGCGGGTGCCCACAACCgtttttgtactcga molybdenum ABC transporter ATPase YP_885443.1
pMSMEG_1047.sgRNA MSMEG_1047 - gatataatctgggaGTCGTTCGCCGACTTCCTGCGCGAgtttttgtactcga nudix hydrolase YP_885444.1
pMSMEG_1048.sgRNA MSMEG_1048 - gatataatctgggaGTTCGGGGATGTCGTCGCCCGCgtttttgtactcga enoylCoA hydratase YP_885445.1
pMSMEG_1049.sgRNA MSMEG_1049 - gatataatctgggaGCCGGACTGGATGAGGTTGAGgtttttgtactcga UbiE/COQ5 family methyltransferase YP_885446.1
pMSMEG_1053.sgRNA MSMEG_1053 - gatataatctgggaAAACTTCCGGCATCATGCCGGgtttttgtactcga hypothetical protein MSMEG_1053 YP_885451.1
pMSMEG_1055.sgRNA MSMEG_1055 - gatataatctgggaACCGCGGCCGATCAGGGTGTTGCgtttttgtactcga hexapeptide transferase YP_885452.1
pMSMEG_1062.sgRNA MSMEG_1062 - gatataatctgggaATCGGTGCGAAACCAGCCCGGTgtttttgtactcga Osuccinylbenzoic acidCoA ligase YP_885458.1
pMSMEG_1063.sgRNA MSMEG_1063 - gatataatctgggaGTTGAGCCAGGCCGCGATCTTGGgtttttgtactcga hypothetical protein MSMEG_1063 YP_885459.1
pMSMEG_1075.sgRNA MSMEG_1075 menB gatataatctgggaACCGGATGAGCCGCTGCACCTgtttttgtactcga naphthoate synthase YP_885471.1
pMSMEG_1103.sgRNA MSMEG_1103 - gatataatctgggaATGTCGATCTGTGCGGCGATGTgtttttgtactcga Osuccinylbenzoate synthase YP_885498.1
pMSMEG_1109.sgRNA MSMEG_1109 - gatataatctgggaGAAGATCCGCGACGACACGTCgtttttgtactcga 2succinyl5enolpyruvyl6hydroxy3cyclohexene1carboxylate synthase YP_885503.1
pMSMEG_1115.sgRNA MSMEG_1115 ubiE gatataatctgggaGGCCGACTTGGTCTCGCGACGgtttttgtactcga ubiquinone/menaquinone biosynthesis methyltransferase YP_885509.1
pMSMEG_1133.sgRNA MSMEG_1133 - gatataatctgggaGATCTGCTCGTCGTTGGCGCCgtttttgtactcga bifunctional short chain isoprenyl diphosphate synthase YP_885527.1
pMSMEG_1229.sgRNA MSMEG_1229 gyrB gatataatctgggaGTCTCGAACACGTCGGGATCGGCgtttttgtactcga DNA gyrase subunit B YP_885621.1
pMSMEG_1340.sgRNA MSMEG_1340 - gatataatctgggaGCTCCGCGGCCGCGTCTTCGTCgtttttgtactcga (3R)hydroxyacylACP dehydratase subunit HadA YP_885728.1
pMSMEG_1341.sgRNA MSMEG_1341 - gatataatctgggaAACTGAACTCACGCAGAGCCAgtttttgtactcga (3R)hydroxyacylACP dehydratase subunit HadB YP_885729.1
pMSMEG_1345.sgRNA MSMEG_1345 nusG gatataatctgggaACCTCTTCGGTGGGCACCTCGACgtttttgtactcga transcription antitermination protein NusG YP_885732.1
pMSMEG_1346.sgRNA MSMEG_1346 rplK gatataatctgggaGGTCGCGGCGTTGTACGCCTTgtttttgtactcga 50S ribosomal protein L11 YP_885733.1
pMSMEG_1347.sgRNA MSMEG_1347 rplA gatataatctgggaGGCGATCGCGGCGTCGAAGTCgtttttgtactcga 50S ribosomal protein L1 YP_885734.1
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