MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_1350.sgRNA MSMEG_1350 - gatataatctgggaGCAACGAGAGCCAGCGCACGCCgtttttgtactcga cyclopropanefattyacylphospholipid synthase YP_885737.1
pMSMEG_1351.sgRNA MSMEG_1351 - gatataatctgggaGCTTGGTGCGGGGACTGGATGACTCgtttttgtactcga cyclopropanefattyacylphospholipid synthase YP_885738.1
pMSMEG_1353.sgRNA MSMEG_1353 - gatataatctgggaGACGTAGGTGGGTCCCAGGTCgtttttgtactcga ABC transporter YP_885740.1
pMSMEG_1364.sgRNA MSMEG_1364 rplJ gatataatctgggaGCGGCAGCGAGCCGGGCCACCgtttttgtactcga 50S ribosomal protein L10 YP_885750.1
pMSMEG_1365.sgRNA MSMEG_1365 rplL gatataatctgggaACAACCACATACAACGTCCAAgtttttgtactcga 50S ribosomal protein L7/L12 YP_885751.1
pMSMEG_1367.sgRNA MSMEG_1367 rpoB gatataatctgggaGAAGGACAGTGACATCGAGCCgtttttgtactcga DNAdirected RNA polymerase subunit beta YP_885753.1
pMSMEG_1368.sgRNA MSMEG_1368 rpoC gatataatctgggaAGTCGGTCCGAAGATCTTCTCgtttttgtactcga DNAdirected RNA polymerase subunit beta\' YP_885754.1
pMSMEG_1398.sgRNA MSMEG_1398 rpsL gatataatctgggaACGCGCGTTTACACCACCACTCCgtttttgtactcga 30S ribosomal protein S12 YP_885784.1
pMSMEG_1399.sgRNA MSMEG_1399 rpsG gatataatctgggaGGTCTTCTCACGACGCTGACGgtttttgtactcga 30S ribosomal protein S7 YP_885785.1
pMSMEG_1400.sgRNA MSMEG_1400 - gatataatctgggaAGGTGGTCAACTCGACCAAGGGgtttttgtactcga Pseudogene
pMSMEG_1401.sgRNA MSMEG_1401 tuf gatataatctgggaGTTCAAATCGGGGAACTTGTCGTgtttttgtactcga elongation factor Tu YP_885786.1
pMSMEG_1435.sgRNA MSMEG_1435 rpsJ gatataatctgggaGTGGGCCCTGTGCCGCTGCCGACgtttttgtactcga 30S ribosomal protein S10 YP_885818.1
pMSMEG_1436.sgRNA MSMEG_1436 rplC gatataatctgggaACCGTGCGCGGCGCCCTGGCCgtttttgtactcga 50S ribosomal protein L3 YP_885819.1
pMSMEG_1437.sgRNA MSMEG_1437 rplD gatataatctgggaAACAGCTCGGCAGGCAGCTCGgtttttgtactcga 50S ribosomal protein L4 YP_885820.1
pMSMEG_1438.sgRNA MSMEG_1438 rplW gatataatctgggaACTTCTCCGAGATGACCGGGGgtttttgtactcga 50S ribosomal protein L23 YP_885821.1
pMSMEG_1439.sgRNA MSMEG_1439 rplB gatataatctgggaGACGCCGTCCTTGTCGTGGCGgtttttgtactcga 50S ribosomal protein L2 YP_885822.1
pMSMEG_1440.sgRNA MSMEG_1440 rpsS gatataatctgggaATCTCCTGAAGAAGGTCGACGTTCAGAAgtttttgtactcga 30S ribosomal protein S19 YP_885823.1
pMSMEG_1441.sgRNA MSMEG_1441 rplV gatataatctgggaGTGGCTGGTGCGCTTGCGGATgtttttgtactcga 50S ribosomal protein L22 YP_885824.1
pMSMEG_1442.sgRNA MSMEG_1442 rpsC gatataatctgggaGGACGCTCACGACGCGGCCGGgtttttgtactcga 30S ribosomal protein S3 YP_885825.1
pMSMEG_1443.sgRNA MSMEG_1443 rplP gatataatctgggaGCTTCTTGGTCAGCGGGCGGTCgtttttgtactcga 50S ribosomal protein L16 YP_885826.1
pMSMEG_1444.sgRNA MSMEG_1444 rpmC gatataatctgggaGTTCGACAGCTGACCGGTGGCCATgtttttgtactcga 50S ribosomal protein L29 YP_885827.1
pMSMEG_1445.sgRNA MSMEG_1445 - gatataatctgggaACGGCAAGATCATCCGGACCACgtttttgtactcga 30S ribosomal protein S17 YP_885828.1
pMSMEG_1465.sgRNA MSMEG_1465 rplN gatataatctgggaGCGTAGCGCCGCGACGAGCCACgtttttgtactcga 50S ribosomal protein L14 YP_885847.1
pMSMEG_1466.sgRNA MSMEG_1466 rplX gatataatctgggaATCCGACACGGGTCGGCTTGCCGgtttttgtactcga 50S ribosomal protein L24 YP_885848.1
pMSMEG_1467.sgRNA MSMEG_1467 rplE gatataatctgggaGAACTGCTTGGGCGACAGACCgtttttgtactcga 50S ribosomal protein L5 YP_885849.1
pMSMEG_1468.sgRNA MSMEG_1468 rpsN gatataatctgggaAGGCGCTGGTCCACAAGGCCAAgtttttgtactcga 30S ribosomal protein S14 YP_885850.1
pMSMEG_1469.sgRNA MSMEG_1469 rpsH gatataatctgggaATTGGCGTTGCGCAGACGTGTgtttttgtactcga 30S ribosomal protein S8 YP_885851.1
pMSMEG_1470.sgRNA MSMEG_1470 rplF gatataatctgggaACTTTCTGCTTGTCGATGCCCGACACgtttttgtactcga 50S ribosomal protein L6 YP_885852.1
pMSMEG_1471.sgRNA MSMEG_1471 rplR gatataatctgggaGCTGCGTCGCCACGCACGCCTGCGgtttttgtactcga 50S ribosomal protein L18 YP_885853.1
pMSMEG_1472.sgRNA MSMEG_1472 rpsE gatataatctgggaGCTGCCGATGAGCGGCACGCGgtttttgtactcga 30S ribosomal protein S5 YP_885854.1
pMSMEG_1473.sgRNA MSMEG_1473 rpmD gatataatctgggaAGAGCCTGCGGACCCTGGGCCTgtttttgtactcga 50S ribosomal protein L30 YP_885855.1
pMSMEG_1474.sgRNA MSMEG_1474 rplO gatataatctgggaACCTGAAGCCGGCTCCCGGAGAgtttttgtactcga 50S ribosomal protein L15 YP_885856.1
pMSMEG_1479.sgRNA MSMEG_1479 - gatataatctgggaACCGCCGCGGTGGCGAACAGGCCgtttttgtactcga methyltransferase, family protein YP_885861.1
pMSMEG_1483.sgRNA MSMEG_1483 secY gatataatctgggaGAGCTGCAGCAGCGCACCGCCgtttttgtactcga preprotein translocase subunit SecY YP_885865.1
pMSMEG_1484.sgRNA MSMEG_1484 adk gatataatctgggaGCGTGCCGTCACCGATGTTCTTgtttttgtactcga adenylate kinase YP_885866.1
pMSMEG_1510.sgRNA MSMEG_1510 - gatataatctgggaGGTCGAATCGTCGTCCAGTGCgtttttgtactcga dTDP4dehydrorhamnose 3,5epimerase YP_885893.1
pMSMEG_1512.sgRNA MSMEG_1512 rfbB gatataatctgggaACGTTGTGCCTGCGCACCGCCTgtttttgtactcga dTDPglucose 4,6dehydratase YP_885894.1
pMSMEG_1519.sgRNA MSMEG_1519 infA gatataatctgggaGCATCTTGCCGCTGATGTGGGgtttttgtactcga translation initiation factor IF1 YP_885901.1
pMSMEG_1520.sgRNA MSMEG_1520 rpmJ gatataatctgggaGTCAACGAGAGATCAGAAGGATCgtttttgtactcga 50S ribosomal protein L36 YP_885902.1
pMSMEG_1521.sgRNA MSMEG_1521 rpsM gatataatctgggaGTTCTTGTCGATGCCCGTCGCAGgtttttgtactcga 30S ribosomal protein S13 YP_885903.1
pMSMEG_1522.sgRNA MSMEG_1522 rpsK gatataatctgggaGCCCTTGAAGCCCACGTGACCgtttttgtactcga 30S ribosomal protein S11 YP_885904.1
pMSMEG_1523.sgRNA MSMEG_1523 rpsD gatataatctgggaGTCGACCTTCACGCCGTTGACgtttttgtactcga 30S ribosomal protein S4 YP_885905.1
pMSMEG_1524.sgRNA MSMEG_1524 rpoA gatataatctgggaACAGGGTGGGACGCTGAGAGAgtttttgtactcga DNAdirected RNA polymerase subunit alpha YP_885906.1
pMSMEG_1525.sgRNA MSMEG_1525 - gatataatctgggaGTAGCCGCCGTTGCGATCGGCgtttttgtactcga 50S ribosomal protein L17 YP_885907.1
pMSMEG_1527.sgRNA MSMEG_1527 truA gatataatctgggaGACGTAGTGGCGGCGCAGCGCgtttttgtactcga tRNA pseudouridine synthase A YP_885908.1
pMSMEG_1556.sgRNA MSMEG_1556 rplM gatataatctgggaATCTGACGGCCGAGCTTGTTGTgtttttgtactcga 50S ribosomal protein L13 YP_885936.1
pMSMEG_1557.sgRNA MSMEG_1557 rpsI gatataatctgggaGAGCTGCTGGTGCACCTTGTTgtttttgtactcga 30S ribosomal protein S9 YP_885937.1
pMSMEG_1559.sgRNA MSMEG_1559 glmM gatataatctgggaGCCGGACTGCTCACCGCCCAGgtttttgtactcga phosphoglucosamine mutase YP_885939.1
pMSMEG_1568.sgRNA MSMEG_1568 glmS gatataatctgggaGCGATCACCAGCGTGCTTCGGTgtttttgtactcga glucosaminefructose6phosphate aminotransferase YP_885947.1
pMSMEG_1573.sgRNA MSMEG_1573 - gatataatctgggaGTGCGTGCGCGCAACAGACGCATgtttttgtactcga carbohydrate kinase YP_885952.1
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