MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

Page of 14
CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_0001.sgRNA MSMEG_0001 dnaN gatataatctgggaGTCCTCGACCGCGAGCGTCGGCAGgtttttgtactcga DNA polymerase III subunit beta YP_884424.1
pMSMEG_0003.sgRNA MSMEG_0003 recF gatataatctgggaATGACTGATACGACGCTGCCGgtttttgtactcga recombination protein F YP_884426.1
pMSMEG_0004.sgRNA MSMEG_0004 - gatataatctgggaGTCGAGGAACTGCAGCCAGAGGTgtttttgtactcga hypothetical protein MSMEG_0004 YP_884427.1
pMSMEG_0005.sgRNA MSMEG_0005 gyrB gatataatctgggaGTTTGCGGACCGCTTCCAAACCCTgtttttgtactcga DNA gyrase subunit B YP_884428.1
pMSMEG_0006.sgRNA MSMEG_0006 gyrA gatataatctgggaATCCAGTCGTGCGTCGAGCAGACgtttttgtactcga DNA gyrase subunit A YP_884429.1
pMSMEG_0007.sgRNA MSMEG_0007 - gatataatctgggaGCTCGCGACCCGCAATGGTCTGGTgtttttgtactcga hypothetical protein MSMEG_0007 YP_884430.1
pMSMEG_0024.sgRNA MSMEG_0024 - gatataatctgggaGTTGAAACCGCCCCCGAACCTGgtttttgtactcga peptidylprolyl cistrans isomerase B YP_884444.1
pMSMEG_0026.sgRNA MSMEG_0026 - gatataatctgggaACCAGTAGCCAGATGAGACCGAgtttttgtactcga septation inhibitor protein YP_884446.1
pMSMEG_0028.sgRNA MSMEG_0028 - gatataatctgggaGTGCCTCGCGGCGGAACCGCAGgtttttgtactcga serinethreonine protein kinase YP_884449.1
pMSMEG_0029.sgRNA MSMEG_0029 - gatataatctgggaGCGTCTCGTCCGGTGCGGCGCCgtttttgtactcga paraaminobenzoate synthase component II YP_884448.1
pMSMEG_0030.sgRNA MSMEG_0030 pknA gatataatctgggaATCGCGACGGTGCGGGCCTCGGCgtttttgtactcga serine/threonine protein kinase YP_884450.1
pMSMEG_0032.sgRNA MSMEG_0032 - gatataatctgggaAGCACCAGAAACGACGCGTACAgtttttgtactcga cell cycle protein, FtsW/RodA/SpoVE family protein YP_884452.1
pMSMEG_0033.sgRNA MSMEG_0033 - gatataatctgggaACGGCTCCTGCAGCGAGATGCCgtttttgtactcga protein phosphatase 2C YP_884453.1
pMSMEG_0034.sgRNA MSMEG_0034 - gatataatctgggaGCCCGTGAGCCCGCGCACGCGAGGAgtttttgtactcga FHA domaincontaining protein YP_884454.1
pMSMEG_0035.sgRNA MSMEG_0035 - gatataatctgggaGCCGTTGGTGGAGTTCAGATCGGgtttttgtactcga FHA domaincontaining protein YP_884455.1
pMSMEG_0151.sgRNA MSMEG_0151 - gatataatctgggaAGGAATCGATTCCGAAGAACGTgtttttgtactcga PntAB protein YP_884567.1
pMSMEG_0234.sgRNA MSMEG_0234 - gatataatctgggaGCCTCGGCGTCGAAGAACGGCGGTTgtttttgtactcga metallopeptidase YP_884649.1
pMSMEG_0244.sgRNA MSMEG_0244 - gatataatctgggaGCGTTCGAGTTTGCGCCGCAGgtttttgtactcga two component response transcriptional regulatory protein prra YP_884659.1
pMSMEG_0250.sgRNA MSMEG_0250 - gatataatctgggaGACAGACTGGCTGCCCTCGTCgtttttgtactcga membrane protein, MmpL family protein YP_884665.1
pMSMEG_0301.sgRNA MSMEG_0301 - gatataatctgggaGGGCCTTCGAGTGGATGGAGTGgtttttgtactcga oxidoreductase, FADbinding YP_884716.1
pMSMEG_0310.sgRNA MSMEG_0310 - gatataatctgggaACGCGCAGGCACGGTCACCGGCCgtttttgtactcga Pseudogene
pMSMEG_0311.sgRNA MSMEG_0311 - gatataatctgggaGGTGTCCTCGTCGACGTGACCgtttttgtactcga glycosyltransferase YP_884725.1
pMSMEG_0317.sgRNA MSMEG_0317 - gatataatctgggaGCACCTGGATGCCCTGCGTATCgtttttgtactcga hypothetical protein MSMEG_0317 YP_884731.1
pMSMEG_0319.sgRNA MSMEG_0319 - gatataatctgggaGCGTCACCATCACGGCGCTGCgtttttgtactcga acyltransferase YP_884732.1
pMSMEG_0359.sgRNA MSMEG_0359 - gatataatctgggaGTTCCACAGATTGAACGCACGGGgtttttgtactcga hypothetical protein MSMEG_0359 YP_884772.1
pMSMEG_0371.sgRNA MSMEG_0371 - gatataatctgggaGGGACAACTCGGGCGGTGAGCAgtttttgtactcga MaoC like domaincontaining protein YP_884784.1
pMSMEG_0380.sgRNA MSMEG_0380 - gatataatctgggaGGTTGCACAACGTCTTCGGCGCgtttttgtactcga MmpS4 protein YP_884793.1
pMSMEG_0384.sgRNA MSMEG_0384 rfbA gatataatctgggaGCTCGTGCCCAATCCTGGTCCgtttttgtactcga glucose1phosphate thymidylyltransferase YP_884797.1
pMSMEG_0392.sgRNA MSMEG_0392 - gatataatctgggaGCCCGAACCGCATACTCGGGTCgtttttgtactcga glycosyl transferase family protein YP_884805.1
pMSMEG_0406.sgRNA MSMEG_0406 - gatataatctgggaACCCTTGTCACGGATGATCTTGCGGAAgtttttgtactcga acylCoA dehydrogenase YP_884819.1
pMSMEG_0415.sgRNA MSMEG_0415 - gatataatctgggaATGGCTGCGTCGAGCCGCGGGTTgtttttgtactcga NADHFMN oxidoreductase YP_884828.1
pMSMEG_0615.sgRNA MSMEG_0615 - gatataatctgggaACCTCGCGCACGGTCTCCTTCTgtttttgtactcga ATPase AAA YP_885026.1
pMSMEG_0616.sgRNA MSMEG_0616 - gatataatctgggaGCACGCCGCCCGGACGGAACAGgtttttgtactcga hypothetical protein MSMEG_0616 YP_885027.1
pMSMEG_0617.sgRNA MSMEG_0617 - gatataatctgggaGTGGCGGTCATCTCCAACATGGCgtttttgtactcga ftsk/SpoIIIE family protein YP_885028.1
pMSMEG_0621.sgRNA MSMEG_0621 - gatataatctgggaAGTTGTACATGATCTGGGACAgtttttgtactcga low molecular weight protein antigen 7 YP_885032.1
pMSMEG_0622.sgRNA MSMEG_0622 - gatataatctgggaATGCTTTCTGGGTTCTTCTCTgtttttgtactcga DNAbinding protein YP_885033.1
pMSMEG_0624.sgRNA MSMEG_0624 - gatataatctgggaGCCGGGCCTCCGGTGCCACGCCgtttttgtactcga subtilase YP_885035.1
pMSMEG_0688.sgRNA MSMEG_0688 - gatataatctgggaGACGTCGTCGACGTCGAACTTgtttttgtactcga aminotransferase YP_885096.1
pMSMEG_0690.sgRNA MSMEG_0690 - gatataatctgggaACGGCGACGGCGAAGAAGTCCTgtttttgtactcga (FeS)binding protein YP_885098.1
pMSMEG_0709.sgRNA MSMEG_0709 dnaK gatataatctgggaACGACGACTGGATCGCCGCCTTgtttttgtactcga molecular chaperone DnaK YP_885116.1
pMSMEG_0710.sgRNA MSMEG_0710 grpE gatataatctgggaACTCGTCGCCCTCCTCGCCGAACgtttttgtactcga cochaperone GrpE YP_885117.1
pMSMEG_0732.sgRNA MSMEG_0732 - gatataatctgggaATTTGCTGATGGCATTGCTGACgtttttgtactcga chaperone ClpB YP_885138.1
pMSMEG_0736.sgRNA MSMEG_0736 - gatataatctgggaGTTGCGGGCGGGCGGCGGCCTgtttttgtactcga hypothetical protein MSMEG_0736 YP_885142.1
pMSMEG_0759.sgRNA MSMEG_0759 purA gatataatctgggaGCTCGACAGCACGTCGAGCTTGGTgtttttgtactcga adenylosuccinate synthetase YP_885165.1
pMSMEG_0789.sgRNA MSMEG_0789 thiE gatataatctgggaGTGTCGGCCAGCACGGCCCGACgtttttgtactcga thiaminephosphate pyrophosphorylase YP_885195.1
pMSMEG_0791.sgRNA MSMEG_0791 thiO gatataatctgggaACCACCTTCTCGGGCAGGCCGTgtttttgtactcga glycine oxidase ThiO YP_885196.1
pMSMEG_0793.sgRNA MSMEG_0793 thiG gatataatctgggaGCGCCGGGCTCGACGCCTGCGCgtttttgtactcga thiazole synthase YP_885198.1
pMSMEG_0825.sgRNA MSMEG_0825 thiD gatataatctgggaGATCCGCTCGGCGCCGAACTCgtttttgtactcga phosphomethylpyrimidine kinase YP_885229.1
pMSMEG_0826.sgRNA MSMEG_0826 thiC gatataatctgggaACCCCGGGCGCAGGCCGTCGCCCAGgtttttgtactcga thiamine biosynthesis protein ThiC YP_885230.1
pMSMEG_0832.sgRNA MSMEG_0832 def gatataatctgggaGTCTCCGGTACCTCGGAGGTCTgtttttgtactcga peptide deformylase YP_885236.1
Next