MSRdb: Mycobacterial Systems Resource



CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_4283.sgRNA MSMEG_4283 sucB gatataatctgggaACCCGGTGGCGGCCTGGTCACgtttttgtactcga dihydrolipoamide acetyltransferase YP_888560.1
pMSMEG_4285.sgRNA MSMEG_4285 lipB gatataatctgggaGATGCCGCACGGGATGATCGAgtttttgtactcga lipoateprotein ligase B YP_888562.1
pMSMEG_4286.sgRNA MSMEG_4286 lipA gatataatctgggaGCCGGCTTGCCGGTGTCGATCTGgtttttgtactcga lipoyl synthase YP_888563.1
pMSMEG_4290.sgRNA MSMEG_4290 glnA gatataatctgggaGTAGAACTCGGCCTCGGCGCCgtttttgtactcga glutamine synthetase YP_888567.1
pMSMEG_4293.sgRNA MSMEG_4293 - gatataatctgggaGCGCCCTCGTCGCGCAGCGTTCgtttttgtactcga bifunctional glutaminesynthetase adenylyltransferase/deadenyltransferase YP_888570.1
pMSMEG_4294.sgRNA MSMEG_4294 glnA gatataatctgggaATGGAGAACTCCGAACTGGTCgtttttgtactcga glutamine synthetase YP_888571.1
pMSMEG_4298.sgRNA MSMEG_4298 panB gatataatctgggaGGCGTCGCCGCGGCCCTGCACgtttttgtactcga 3methyl2oxobutanoate hydroxymethyltransferase YP_888575.1
pMSMEG_4305.sgRNA MSMEG_4305 - gatataatctgggaGCCAGGTCCAGGTGCAGGCGGTgtttttgtactcga bifunctional RNase H/acid phosphatase YP_888582.1
pMSMEG_4311.sgRNA MSMEG_4311 - gatataatctgggaGCGCCAGCCACTGCGGGCGCAgtttttgtactcga hypothetical protein MSMEG_4311 YP_888587.1
pMSMEG_4323.sgRNA MSMEG_4323 aceE gatataatctgggaGCCGCCGGGATGGCTCTTGCCGCGgtttttgtactcga pyruvate dehydrogenase subunit E1 YP_888598.1
pMSMEG_4324.sgRNA MSMEG_4324 - gatataatctgggaGCTGCGACGCGTCGAGCTCGGCgtttttgtactcga hypothetical protein MSMEG_4324 YP_888599.1
pMSMEG_4325.sgRNA MSMEG_4325 - gatataatctgggaGATCCCGGCGGACTCCGCATTgtttttgtactcga malonyl CoAACP transacylase YP_888600.1
pMSMEG_4326.sgRNA MSMEG_4326 acpP gatataatctgggaACCGCCACGCTGCGTGATCGCTTgtttttgtactcga acyl carrier protein YP_888601.1
pMSMEG_4327.sgRNA MSMEG_4327 - gatataatctgggaATCGGCACCGGGCTCGGCGGCGGgtttttgtactcga 3oxoacylACP synthase YP_888602.1
pMSMEG_4329.sgRNA MSMEG_4329 - gatataatctgggaACACCGGAGCGGTCACGCTCGTgtttttgtactcga propionylCoA carboxylase subunit beta YP_888604.1
pMSMEG_4340.sgRNA MSMEG_4340 - gatataatctgggaGCTTCGACACGTTCAACGCCACgtttttgtactcga NAD/mycothioldependent formaldehyde dehydrogenase YP_888615.1
pMSMEG_4383.sgRNA MSMEG_4383 - gatataatctgggaGGGCGCGACCGTGCCGTCGCTGgtttttgtactcga MmpL5 protein YP_888658.1
pMSMEG_4415.sgRNA MSMEG_4415 tal gatataatctgggaACCTTGCCGACATCTTCGAGACCTTgtttttgtactcga transaldolase YP_888690.1
pMSMEG_4437.sgRNA MSMEG_4437 ctaD gatataatctgggaGCCGAGTGCGATCGACGCACCGAgtttttgtactcga cytochrome C oxidase subunit I YP_888712.1
pMSMEG_4446.sgRNA MSMEG_4446 - gatataatctgggaGGAGCCCGAACATGCATCTGGAgtttttgtactcga Pseudogene
pMSMEG_4467.sgRNA MSMEG_4467 - gatataatctgggaGGCCGACACCACCGGAAGCGCCgtttttgtactcga ABC transporter permease YP_888740.1
pMSMEG_4479.sgRNA MSMEG_4479 - gatataatctgggaAGAAACGTCGGTGCTCTCCGTgtttttgtactcga hypothetical protein MSMEG_4479 YP_888751.1
pMSMEG_4482.sgRNA MSMEG_4482 dnaG gatataatctgggaACACGTAGATCAGTTCGCCGCGgtttttgtactcga DNA primase YP_888754.1
pMSMEG_4485.sgRNA MSMEG_4485 glyS gatataatctgggaAGGTTCGCAACGGTGTCGATGgtttttgtactcga glycyltRNA synthetase YP_888757.1
pMSMEG_4490.sgRNA MSMEG_4490 uppS gatataatctgggaGACGGGCCAGGTCGACTTGTCgtttttgtactcga UDP pyrophosphate synthase YP_888762.1
pMSMEG_4491.sgRNA MSMEG_4491 recO gatataatctgggaGGCGCATGGCAACCACACGGGGgtttttgtactcga DNA repair protein RecO YP_888763.1
pMSMEG_4493.sgRNA MSMEG_4493 era gatataatctgggaGCCCTTCTGGCTGTCGCGCTCGACGTgtttttgtactcga GTPbinding protein Era YP_888765.1
pMSMEG_4496.sgRNA MSMEG_4496 - gatataatctgggaACATGCAGGAGGCGTACGCCGAgtttttgtactcga metalloprotease YP_888768.1
pMSMEG_4533.sgRNA MSMEG_4533 - gatataatctgggaACGGGTGACGTCGGGTTCGACgtttttgtactcga sulfatebinding protein YP_888805.1
pMSMEG_4567.sgRNA MSMEG_4567 - gatataatctgggaACATCGGCGCGATGGTGGTGATgtttttgtactcga hypothetical protein MSMEG_4567 YP_888837.1
pMSMEG_4571.sgRNA MSMEG_4571 rpsT gatataatctgggaACTTCGGTAACCCCAGCTAGAGATgtttttgtactcga 30S ribosomal protein S20 YP_888840.1
pMSMEG_4572.sgRNA MSMEG_4572 - gatataatctgggaGCACCACGTGCGGCTCCCCCGgtttttgtactcga hypothetical protein MSMEG_4572 YP_888841.1
pMSMEG_4579.sgRNA MSMEG_4579 - gatataatctgggaACATCCGCTGGCGACTCGACGTgtttttgtactcga phosphoglycerate mutase YP_888848.1
pMSMEG_4581.sgRNA MSMEG_4581 nadD gatataatctgggaGTCGATGTCGACCCGGCTGACgtttttgtactcga nicotinic acid mononucleotide adenylyltransferase YP_888850.1
pMSMEG_4621.sgRNA MSMEG_4621 proB gatataatctgggaGCAGGTCGACCACGTCGCCGCCgtttttgtactcga gammaglutamyl kinase YP_888886.1
pMSMEG_4623.sgRNA MSMEG_4623 obgE gatataatctgggaGTGCGACGACGTGGGGATGGAAgtttttgtactcga GTPase ObgE YP_888887.1
pMSMEG_4624.sgRNA MSMEG_4624 rpmA gatataatctgggaGAGTCGCGACCGTTGCGAGAGgtttttgtactcga 50S ribosomal protein L27 YP_888888.1
pMSMEG_4625.sgRNA MSMEG_4625 rplU gatataatctgggaGGAGGCGTTTCGACATGGCACAgtttttgtactcga 50S ribosomal protein L21 YP_888889.1
pMSMEG_4626.sgRNA MSMEG_4626 - gatataatctgggaGTCGCCGGAGACGATCAGGCTgtttttgtactcga ribonuclease, Rne/Rng family protein YP_888890.1
pMSMEG_4629.sgRNA MSMEG_4629 - gatataatctgggaACTCGTCGCGGATCAGGCCGTGgtttttgtactcga Pseudogene
pMSMEG_4630.sgRNA MSMEG_4630 valS gatataatctgggaGTCGAGAAGGGCCACAGCGCCGAgtttttgtactcga valyltRNA synthetase YP_888893.1
pMSMEG_4671.sgRNA MSMEG_4671 clpX gatataatctgggaGCTCTTGTCGCGGCGCTCGCTGgtttttgtactcga ATPdependent protease ATPbinding subunit ClpX YP_888934.1
pMSMEG_4672.sgRNA MSMEG_4672 clpP gatataatctgggaACGCGTCGTCCACCTGCACGCCgtttttgtactcga ATPdependent Clp protease proteolytic subunit YP_888935.1
pMSMEG_4673.sgRNA MSMEG_4673 clpP gatataatctgggaGTCGTCGTCCACTTGGGAACCgtttttgtactcga ATPdependent Clp protease proteolytic subunit YP_888936.1
pMSMEG_4684.sgRNA MSMEG_4684 - gatataatctgggaGGTCTTCTCGGCCGCGGCGATgtttttgtactcga ribose5phosphate isomerase B YP_888945.1
pMSMEG_4700.sgRNA MSMEG_4700 - gatataatctgggaGACACCGATCTTCGCGCCAGGgtttttgtactcga ABC transporter ATPbinding protein YP_888961.1
pMSMEG_4722.sgRNA MSMEG_4722 - gatataatctgggaGGTGTACTCGGTGTCGATCCAgtttttgtactcga shortchain dehydrogenase YP_888982.1
pMSMEG_4756.sgRNA MSMEG_4756 acpS gatataatctgggaACGCGACGATCCTGGTGACCGGTgtttttgtactcga 4\'phosphopantetheinyl transferase YP_889014.1
pMSMEG_4757.sgRNA MSMEG_4757 - gatataatctgggaGCAGCACGGTGCGCACGTCGGAgtttttgtactcga fatty acid synthase YP_889015.1
pMSMEG_4898.sgRNA MSMEG_4898 - gatataatctgggaGCGCACGAACTTGTAGTACGCgtttttgtactcga hypothetical protein MSMEG_4898 YP_889153.1
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