MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_4900.sgRNA MSMEG_4900 - gatataatctgggaGAAGTGCTCGACGATGATGCCgtttttgtactcga Pks14 protein YP_889154.1
pMSMEG_4903.sgRNA MSMEG_4903 murI gatataatctgggaGCACACCGTCCAACGTCGGGCCgtttttgtactcga glutamate racemase YP_889158.1
pMSMEG_4909.sgRNA MSMEG_4909 - gatataatctgggaGCGTTCGTCGTCGCCGACGCCGGgtttttgtactcga hypothetical protein MSMEG_4909 YP_889164.1
pMSMEG_4917.sgRNA MSMEG_4917 - gatataatctgggaGCCGCATGCCCGGCGGCGACCAgtttttgtactcga hypothetical protein MSMEG_4917 YP_889173.1
pMSMEG_4932.sgRNA MSMEG_4932 murA gatataatctgggaGGGGCGCTCGTACTGCACGACgtttttgtactcga UDPNacetylglucosamine 1carboxyvinyltransferase YP_889184.1
pMSMEG_4934.sgRNA MSMEG_4934 - gatataatctgggaATCTCGCAGGACTACATCGACCGCCTgtttttgtactcga ATP:cob(I)alamin adenosyltransferase YP_889185.1
pMSMEG_4935.sgRNA MSMEG_4935 atpC gatataatctgggaGACGGTCTCCTCGGTCACCGAgtttttgtactcga F0F1 ATP synthase subunit epsilon YP_889187.1
pMSMEG_4936.sgRNA MSMEG_4936 atpD gatataatctgggaATACGGTTGATCATCTCCTGGAgtttttgtactcga F0F1 ATP synthase subunit beta YP_889188.1
pMSMEG_4937.sgRNA MSMEG_4937 atpG gatataatctgggaGTTCTCGTAGGTCGGGCGCTCgtttttgtactcga F0F1 ATP synthase subunit gamma YP_889189.1
pMSMEG_4938.sgRNA MSMEG_4938 atpA gatataatctgggaGACACGCTCTGGCGCTGCACCgtttttgtactcga F0F1 ATP synthase subunit alpha YP_889190.1
pMSMEG_4941.sgRNA MSMEG_4941 atpE gatataatctgggaGCTTCCACCAGACCGACGGTGATgtttttgtactcga F0F1 ATP synthase subunit C YP_889193.1
pMSMEG_4942.sgRNA MSMEG_4942 atpB gatataatctgggaGGAGGTGACCTTGGCCCGCAGgtttttgtactcga F0F1 ATP synthase subunit A YP_889194.1
pMSMEG_4947.sgRNA MSMEG_4947 - gatataatctgggaACCATCGCGGTCTGGCCCGGATCgtttttgtactcga glycosyl trasferase YP_889199.1
pMSMEG_4948.sgRNA MSMEG_4948 - gatataatctgggaGACAAGGCTCAGCGCGCCCGGgtttttgtactcga Sua5/YciO/YrdC/YwlC family protein YP_889200.1
pMSMEG_4949.sgRNA MSMEG_4949 - gatataatctgggaGATCGACGATCACCGCGTCGCgtttttgtactcga HemK family modification methylase YP_889201.1
pMSMEG_4950.sgRNA MSMEG_4950 prfA gatataatctgggaGTGATCGGCGATGCGGTTCTCgtttttgtactcga peptide chain release factor 1 YP_889202.1
pMSMEG_4954.sgRNA MSMEG_4954 rho gatataatctgggaGCTCGATGTTGCGGGCGGCGCCgtttttgtactcga transcription termination factor Rho YP_889206.1
pMSMEG_4955.sgRNA MSMEG_4955 thrB gatataatctgggaGCGCACCACCAGACCCGGCGCgtttttgtactcga homoserine kinase YP_889207.1
pMSMEG_4956.sgRNA MSMEG_4956 thrC gatataatctgggaACCGAGTTCACCAGCGCGATGGTgtttttgtactcga threonine synthase YP_889208.1
pMSMEG_4957.sgRNA MSMEG_4957 - gatataatctgggaGGCACCGCCGGCGCCTTGACCgtttttgtactcga homoserine dehydrogenase YP_889209.1
pMSMEG_4958.sgRNA MSMEG_4958 lysA gatataatctgggaATTGACCCTTCACCTCCAGGCgtttttgtactcga diaminopimelate decarboxylase YP_889210.1
pMSMEG_4959.sgRNA MSMEG_4959 argS gatataatctgggaGCGGCAGCACGCGGCACGAGTCgtttttgtactcga arginyltRNA synthetase YP_889211.1
pMSMEG_5004.sgRNA MSMEG_5004 - gatataatctgggaACGACACCGCGATGCGTGCCATgtttttgtactcga DNA repair exonuclease YP_889255.1
pMSMEG_5041.sgRNA MSMEG_5041 - gatataatctgggaGGAACACCATCGGCCGGCTCGgtttttgtactcga acyltransferase YP_889291.1
pMSMEG_5049.sgRNA MSMEG_5049 kgd gatataatctgggaACTTGTCACGGCTTGCCGGACAgtttttgtactcga alphaketoglutarate decarboxylase YP_889299.1
pMSMEG_5058.sgRNA MSMEG_5058 - gatataatctgggaACCGACATCGGTACGCGTCGCCGGgtttttgtactcga ABC transporter ATPbinding protein YP_889308.1
pMSMEG_5068.sgRNA MSMEG_5068 - gatataatctgggaACCCGTGCCGGGCGGCAGATCGAgtttttgtactcga Mrp protein YP_889318.1
pMSMEG_5070.sgRNA MSMEG_5070 - gatataatctgggaAAGCCGAGGCCGCTTGCGCTGgtttttgtactcga trypsin YP_889320.1
pMSMEG_5073.sgRNA MSMEG_5073 - gatataatctgggaGCCCCGGCCCGACGCCGGCCTCgtttttgtactcga Omethyltransferase, family protein 3 YP_889323.1
pMSMEG_5084.sgRNA MSMEG_5084 - gatataatctgggaGCTGACCTTGAGCGGCCTGCGgtttttgtactcga glucosyl3phosphoglycerate synthase YP_889334.1
pMSMEG_5103.sgRNA MSMEG_5103 dapE gatataatctgggaGACGTCGCGATCCGGCGCGAAgtttttgtactcga succinyldiaminopimelate desuccinylase YP_889352.1
pMSMEG_5104.sgRNA MSMEG_5104 - gatataatctgggaGCTCGGGAGCGGGAAACCAGGTGTgtttttgtactcga tetrahydropicolinate succinylase YP_889353.1
pMSMEG_5122.sgRNA MSMEG_5122 - gatataatctgggaGCCCGGCGAGCCGAGTTCGGCgtttttgtactcga ferredoxin YP_889369.1
pMSMEG_5129.sgRNA MSMEG_5129 - gatataatctgggaATCTACTCCAGATCAAGCCCGGgtttttgtactcga Nacetyl1DmyoInosityl2amino2deoxyalpha Dglucopyranoside deacetylase MshB YP_889375.1
pMSMEG_5130.sgRNA MSMEG_5130 - gatataatctgggaACGCCACGCCGGGTACGGCTGgtttttgtactcga extracellular solutebinding protein YP_889376.1
pMSMEG_5149.sgRNA MSMEG_5149 pimE gatataatctgggaGCCGTACTGCTTGCCCAGCAGCGCgtttttgtactcga mannosyltransferase YP_889396.1
pMSMEG_5224.sgRNA MSMEG_5224 ispH gatataatctgggaACCGTCGGTGCGACGCCGTGCGCgtttttgtactcga 4hydroxy3methylbut2enyl diphosphate reductase YP_889470.1
pMSMEG_5227.sgRNA MSMEG_5227 xseB gatataatctgggaGTTGGTCGCCGTGGTGCAGCAGgtttttgtactcga exodeoxyribonuclease VII small subunit YP_889473.1
pMSMEG_5240.sgRNA MSMEG_5240 fumC gatataatctgggaGCTCGAGGCCGCGGAACGAGATgtttttgtactcga fumarate hydratase YP_889486.1
pMSMEG_5248.sgRNA MSMEG_5248 - gatataatctgggaGAACACGGCGTGCGAGCGCTCgtttttgtactcga acyl YP_889494.1
pMSMEG_5252.sgRNA MSMEG_5252 coaA gatataatctgggaGATGCGCGCGTCGACGTACACgtttttgtactcga pantothenate kinase YP_889497.1
pMSMEG_5271.sgRNA MSMEG_5271 - gatataatctgggaACCGAAGGTGGCGACCACCGCgtttttgtactcga esterase YP_889517.1
pMSMEG_5328.sgRNA MSMEG_5328 - gatataatctgggaGCCAGACGGTTCTGGTGCTGCACgtttttgtactcga hypothetical protein MSMEG_5328 YP_889574.1
pMSMEG_5413.sgRNA MSMEG_5413 - gatataatctgggaGCTGATCGACAGCGCGATACCGTgtttttgtactcga exopolyphosphatase YP_889654.1
pMSMEG_5414.sgRNA MSMEG_5414 - gatataatctgggaGTCAGCACGCACACGACGGCGGgtttttgtactcga septum formation initiator subfamily protein YP_889655.1
pMSMEG_5415.sgRNA MSMEG_5415 eno gatataatctgggaGTCGTGACCCAGGAGCCCGAACCgtttttgtactcga phosphopyruvate hydratase YP_889656.1
pMSMEG_5426.sgRNA MSMEG_5426 glmU gatataatctgggaATGCGCGTGCCGGCTCCGGCGGgtttttgtactcga bifunctional Nacetylglucosamine1phosphate uridyltransferase/glucosamine1phosphate acetyltransferase YP_889665.1
pMSMEG_5427.sgRNA MSMEG_5427 - gatataatctgggaGCATGTGGTCCACCGGGCCGTCgtttttgtactcga ribosephosphate pyrophosphokinase YP_889666.1
pMSMEG_5431.sgRNA MSMEG_5431 - gatataatctgggaGCTCGGTGCCCTCGATGTCCAGGGgtttttgtactcga 50S ribosomal protein L25 YP_889670.1
pMSMEG_5432.sgRNA MSMEG_5432 pth gatataatctgggaGATCTGCTGCGGTGGCACCGAgtttttgtactcga peptidyltRNA hydrolase YP_889671.1
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