MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_3790.sgRNA MSMEG_3790 - gatataatctgggaGTCGGGATCGCTGATGACCGGGATgtttttgtactcga TrmH family RNA methyltransferase YP_888083.1
pMSMEG_3791.sgRNA MSMEG_3791 rplT gatataatctgggaGATGCGGGAGATCCACAGCTTgtttttgtactcga 50S ribosomal protein L20 YP_888084.1
pMSMEG_3792.sgRNA MSMEG_3792 rpmI gatataatctgggaGTCAAGCCCGCTACCTGCACCGAgtttttgtactcga 50S ribosomal protein L35 YP_888085.1
pMSMEG_3793.sgRNA MSMEG_3793 infC gatataatctgggaACCTTGACCTTCGACCCGGCCTCgtttttgtactcga translation initiation factor IF3 YP_888086.1
pMSMEG_3808.sgRNA MSMEG_3808 uvrA gatataatctgggaAGAACCGCATACAGACCGACGAgtttttgtactcga excinuclease ABC subunit A YP_888102.1
pMSMEG_3811.sgRNA MSMEG_3811 - gatataatctgggaGCCTTGGCGCGGTCGTTGGCCTCACgtttttgtactcga universal stress protein family protein YP_888104.1
pMSMEG_3831.sgRNA MSMEG_3831 - gatataatctgggaGCCTGGAGGTTACGTGCGAACGgtttttgtactcga Pseudogene
pMSMEG_3833.sgRNA MSMEG_3833 rpsA gatataatctgggaATTTGATGGTCTTGTCGATGGCGGgtttttgtactcga 30S ribosomal protein S1 YP_888124.1
pMSMEG_3839.sgRNA MSMEG_3839 - gatataatctgggaACGGCCTGCTCGTCGACGCCGgtttttgtactcga DNA polymerase I YP_888130.1
pMSMEG_3858.sgRNA MSMEG_3858 - gatataatctgggaGCTCCTCGACGGACCGGCGCTCCAgtttttgtactcga hypothetical protein MSMEG_3858 YP_888149.1
pMSMEG_3860.sgRNA MSMEG_3860 - gatataatctgggaGATCCACGGCAGCAGCGGCACgtttttgtactcga polyprenolmonophosphomannose synthase Ppm1 YP_888151.1
pMSMEG_3863.sgRNA MSMEG_3863 - gatataatctgggaACCGGCTGCTGGTGATCACCGAgtttttgtactcga pyridoxamine 5\'phosphate oxidase YP_888154.1
pMSMEG_3886.sgRNA MSMEG_3886 tatC gatataatctgggaATCCCGAACACGAGCAGCAGGTTGAgtttttgtactcga twin argininetargeting protein translocase TatC YP_888177.1
pMSMEG_3889.sgRNA MSMEG_3889 - gatataatctgggaATCTGGTGGCCCGGTACGGCGCgtttttgtactcga Pseudogene
pMSMEG_3894.sgRNA MSMEG_3894 - gatataatctgggaGCCTGTTCGGGCGATATGAAGTAgtttttgtactcga proteasome subunit alpha YP_888184.1
pMSMEG_3895.sgRNA MSMEG_3895 - gatataatctgggaGGCCTGCCTGCTGAGCAGTTCgtttttgtactcga proteasome subunit beta YP_888185.1
pMSMEG_3902.sgRNA MSMEG_3902 - gatataatctgggaATCATCGCGATTTTCGACAGCGgtttttgtactcga ATPase AAA YP_888192.1
pMSMEG_4180.sgRNA MSMEG_4180 hisG gatataatctgggaATCGCGATGTCCTTGGGCCGCAGgtttttgtactcga ATP phosphoribosyltransferase YP_888459.1
pMSMEG_4181.sgRNA MSMEG_4181 hisE gatataatctgggaACAAGGCATCGAAAGTCTTCAgtttttgtactcga phosphoribosylATP pyrophosphatase YP_888460.1
pMSMEG_4189.sgRNA MSMEG_4189 cysS gatataatctgggaACCGGTCCTCGCGGTAGTGCCCgtttttgtactcga cysteinyltRNA synthetase YP_888468.1
pMSMEG_4198.sgRNA MSMEG_4198 pyrD gatataatctgggaGGCCGCGAGTCCCAGCGGTCCgtttttgtactcga dihydroorotate dehydrogenase 2 YP_888477.1
pMSMEG_4199.sgRNA MSMEG_4199 - gatataatctgggaGCGGTCATCCACGGCACCTACGAgtttttgtactcga hypothetical protein MSMEG_4199 YP_888478.1
pMSMEG_4217.sgRNA MSMEG_4217 - gatataatctgggaGTCAGCTCGTTCTCAACCAGATgtttttgtactcga DivIVA protein YP_888494.1
pMSMEG_4222.sgRNA MSMEG_4222 ftsZ gatataatctgggaGCTGCGCCGCTTGCCCTCGAAgtttttgtactcga cell division protein FtsZ YP_888500.1
pMSMEG_4226.sgRNA MSMEG_4226 murC gatataatctgggaGGTGTAGGCCTGCTCGCTGCCgtttttgtactcga UDPNacetylmuramateLalanine ligase YP_888503.1
pMSMEG_4227.sgRNA MSMEG_4227 murG gatataatctgggaATCCTTCGACGGTTTGCGTGGCAgtttttgtactcga UDPdiphosphomuramoylpentapeptide betaN acetylglucosaminyltransferase YP_888504.1
pMSMEG_4228.sgRNA MSMEG_4228 ftsW gatataatctgggaGGCCAGCTCCGAGGGCTGCATgtttttgtactcga cell division protein FtsW YP_888505.1
pMSMEG_4229.sgRNA MSMEG_4229 murD gatataatctgggaGACTCATTTGTCCCAAGCACCgtttttgtactcga UDPNacetylmuramoylLalanylDglutamate synthetase YP_888506.1
pMSMEG_4230.sgRNA MSMEG_4230 mraY gatataatctgggaACCAGGCGCTGACCACCACGACgtttttgtactcga phosphoNacetylmuramoylpentapeptidetransferase YP_888507.1
pMSMEG_4231.sgRNA MSMEG_4231 - gatataatctgggaGTCGGGCCGACATCTCCAACACgtttttgtactcga UDPNacetylmuramoyltripeptideDalanylDalanine ligase YP_888508.1
pMSMEG_4232.sgRNA MSMEG_4232 murE gatataatctgggaGAAGTAGTCCGCCATCGTCGGgtttttgtactcga UDPNacetylmuramoylalanylDglutamate2,6diaminopimelate ligase YP_888509.1
pMSMEG_4233.sgRNA MSMEG_4233 - gatataatctgggaGCCCGAACTTGCGCAGCATCTCgtttttgtactcga penicillin binding protein transpeptidase domaincontaining protein YP_888510.1
pMSMEG_4236.sgRNA MSMEG_4236 mraZ gatataatctgggaGCAGAGTTTCATCGGTGGCCGCgtttttgtactcga cell division protein MraZ YP_888513.1
pMSMEG_4241.sgRNA MSMEG_4241 - gatataatctgggaACCGAGAGCAGCAGCGGCGCCAGgtttttgtactcga integral membrane protein YP_888518.1
pMSMEG_4242.sgRNA MSMEG_4242 - gatataatctgggaACTTGACCACGTGGCCCGCGTCGTCgtttttgtactcga transcriptional regulatory protein YP_888519.1
pMSMEG_4243.sgRNA MSMEG_4243 - gatataatctgggaGTTACGAGTCCCGGGCGGCAGCgtttttgtactcga serine/threonine protein kinase YP_888520.1
pMSMEG_4244.sgRNA MSMEG_4244 - gatataatctgggaATCGCGGAGCATCTCCGCGACgtttttgtactcga 3deoxy7phosphoheptulonate synthase YP_888521.1
pMSMEG_4248.sgRNA MSMEG_4248 - gatataatctgggaACCCGCCAGGCCGTCGAAGCGgtttttgtactcga 1acylglycerol3phosphate Oacyltransferase YP_888525.1
pMSMEG_4253.sgRNA MSMEG_4253 - gatataatctgggaACTGGATCCCGCCGCGTCGCGgtttttgtactcga glycoside hydrolase family protein YP_888531.1
pMSMEG_4258.sgRNA MSMEG_4258 trpD gatataatctgggaGCCGGAAGCGGATGGGAACGGCgtttttgtactcga anthranilate phosphoribosyltransferase YP_888536.1
pMSMEG_4260.sgRNA MSMEG_4260 - gatataatctgggaGTACATCGCAAACAGTCCAGCgtttttgtactcga cytochrome C oxidase subunit 3 YP_888537.1
pMSMEG_4261.sgRNA MSMEG_4261 - gatataatctgggaGCGACTGCCAGACCGATCAGCAgtttttgtactcga ubiquinolcytochrome C reductase cytochrome C subunit YP_888538.1
pMSMEG_4262.sgRNA MSMEG_4262 - gatataatctgggaGAAAGGCTGGTACTCCCAAGGgtttttgtactcga ubiquinolcytochrome C reductase ironsulfur subunit YP_888539.1
pMSMEG_4263.sgRNA MSMEG_4263 - gatataatctgggaGACGTGTGCCATCGACGGATCgtttttgtactcga ubiquinolcytochrome C reductase cytochrome b subunit YP_888540.1
pMSMEG_4268.sgRNA MSMEG_4268 - gatataatctgggaGCCTTCTTGCGGTGGAAGGCGCTGGTgtttttgtactcga cytochrome C oxidase subunit 2 YP_888545.1
pMSMEG_4269.sgRNA MSMEG_4269 asnB gatataatctgggaGATGCCGAACGGGTCACGCGCgtttttgtactcga asparagine synthase YP_888546.1
pMSMEG_4270.sgRNA MSMEG_4270 - gatataatctgggaGTGGATGACCAGATCGTCGACgtttttgtactcga adenosine kinase YP_888547.1
pMSMEG_4272.sgRNA MSMEG_4272 - gatataatctgggaACGATCGACTTCGTCGACACCATgtttttgtactcga HesB/YadR/YfhF family protein YP_888549.1
pMSMEG_4276.sgRNA MSMEG_4276 ilvE gatataatctgggaATCGGTCGCCGGATTCGTGTTGgtttttgtactcga branchedchain amino acid aminotransferase YP_888554.1
pMSMEG_4278.sgRNA MSMEG_4278 gcvT gatataatctgggaGGTGTTGGCGGCGTTGGGCACgtttttgtactcga glycine cleavage system aminomethyltransferase T YP_888555.1
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