MSRdb: Mycobacterial Systems Resource



CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_1575.sgRNA MSMEG_1575 alr gatataatctgggaGTTCGCCGTTGGCGCCCGGGCCGAgtttttgtactcga alanine racemase YP_885954.1
pMSMEG_1579.sgRNA MSMEG_1579 rimI gatataatctgggaACTACATCGCGGCCCGCTGTGgtttttgtactcga ribosomalproteinalanine acetyltransferase YP_885958.1
pMSMEG_1582.sgRNA MSMEG_1582 groES gatataatctgggaGCCCCGGCCGCTGGGATGAGGACGGgtttttgtactcga cochaperonin GroES YP_885961.1
pMSMEG_1601.sgRNA MSMEG_1601 - gatataatctgggaGCGCGTCACCTCACCGACAACTTgtttttgtactcga hypothetical protein MSMEG_1601 YP_885980.1
pMSMEG_1602.sgRNA MSMEG_1602 guaB gatataatctgggaGTCCTCCGACAGCACGTCGTCgtttttgtactcga inosine 5\'monophosphate dehydrogenase YP_885981.1
pMSMEG_1603.sgRNA MSMEG_1603 - gatataatctgggaACGGGTGCGCCGCGGCCGCCGGgtttttgtactcga inosine 5monophosphate dehydrogenase YP_885982.1
pMSMEG_1610.sgRNA MSMEG_1610 guaA gatataatctgggaGCCGAGCGCCTGCGCCATGGCgtttttgtactcga GMP synthase YP_885988.1
pMSMEG_1647.sgRNA MSMEG_1647 - gatataatctgggaGGCTTCGACGACGTTGGTCAGgtttttgtactcga bifunctional 5,10methylenetetrahydrofolate dehydrogenase/ 5,10methylenetetrahydrofolate cyclohydrolase YP_886023.1
pMSMEG_1651.sgRNA MSMEG_1651 metX gatataatctgggaGGGAGTCGCCGGTGAGCGCGTgtttttgtactcga homoserine Oacetyltransferase YP_886028.1
pMSMEG_1654.sgRNA MSMEG_1654 - gatataatctgggaACAAGGACGCGTTCGCCAAGCAgtttttgtactcga isocitrate dehydrogenase YP_886031.1
pMSMEG_1657.sgRNA MSMEG_1657 trpS gatataatctgggaGTCCGATGTGGGCTGAGCGCCgtttttgtactcga tryptophanyltRNA synthetase YP_886034.1
pMSMEG_1669.sgRNA MSMEG_1669 sdhB gatataatctgggaACAGATATGACCGAAACGAAGAgtttttgtactcga succinate dehydrogenase ironsulfur subunit YP_886045.1
pMSMEG_1670.sgRNA MSMEG_1670 sdhA gatataatctgggaAGCCCATCCGCGGCCTGCCCGTgtttttgtactcga succinate dehydrogenase flavoprotein subunit YP_886046.1
pMSMEG_1671.sgRNA MSMEG_1671 - gatataatctgggaACGTGAAACCGGTCGCCAGCAgtttttgtactcga succinate dehydrogenase hydrophobic membrane anchor protein SdhD YP_886047.1
pMSMEG_1673.sgRNA MSMEG_1673 cdd gatataatctgggaGGACGGTGACCGGATGCAATGTgtttttgtactcga cytidine deaminase YP_886049.1
pMSMEG_1734.sgRNA MSMEG_1734 - gatataatctgggaAGGGCGCCGAGTTGGGGATCCAgtttttgtactcga hypothetical protein MSMEG_1734 YP_886106.1
pMSMEG_1736.sgRNA MSMEG_1736 - gatataatctgggaGCTTGCGCCCGTTGCTGTGGCCCgtttttgtactcga glycerol3phosphate dehydrogenase YP_886108.1
pMSMEG_1807.sgRNA MSMEG_1807 - gatataatctgggaGGGCGAAGTCGGCGTTCTCGGgtttttgtactcga acetyl/propionylcoenzyme A carboxylase subunit alpha YP_886179.1
pMSMEG_1809.sgRNA MSMEG_1809 - gatataatctgggaACGTCGATCAGCGGCTGCTTGCgtttttgtactcga thiosulfate sulfurtransferase YP_886181.1
pMSMEG_1811.sgRNA MSMEG_1811 maf gatataatctgggaGCTGACCACGACACGCCCACGGgtttttgtactcga Maflike protein YP_886183.1
pMSMEG_1813.sgRNA MSMEG_1813 - gatataatctgggaGCCCGAGGCCTTGATGTTGTTgtttttgtactcga propionylCoA carboxylase subunit beta YP_886185.1
pMSMEG_1818.sgRNA MSMEG_1818 - gatataatctgggaGCGGCGCCGAATCGGGCCCGCTgtttttgtactcga membrane flanked domaincontaining protein YP_886188.1
pMSMEG_1819.sgRNA MSMEG_1819 purK gatataatctgggaGGGACTGCGGAAGAATTGGTTgtttttgtactcga phosphoribosylaminoimidazole carboxylase ATPase subunit YP_886190.1
pMSMEG_1820.sgRNA MSMEG_1820 purE gatataatctgggaACCGCAGGGCTGCGTCCTTTTgtttttgtactcga phosphoribosylaminoimidazole carboxylase catalytic subunit YP_886191.1
pMSMEG_1825.sgRNA MSMEG_1825 rfbD gatataatctgggaGCCACGCCCGCGTTGGCGGCGTgtttttgtactcga dTDP4dehydrorhamnose reductase YP_886196.1
pMSMEG_1826.sgRNA MSMEG_1826 - gatataatctgggaGCCAGCGGTCGGACGCCTCGAgtttttgtactcga dTDPRhA:aDGlcNAcdiphosphoryl polyprenol, a3Lrhamnosyl transferase YP_886197.1
pMSMEG_1830.sgRNA MSMEG_1830 cofD gatataatctgggaATCCCCATTCCTCCAACCACAgtttttgtactcga LPPG:FO 2phosphoLlactate transferase YP_886200.1
pMSMEG_1831.sgRNA MSMEG_1831 - gatataatctgggaGGGCCATGTCGTCGAGCATGCCgtttttgtactcga transcription factor WhiB YP_886201.1
pMSMEG_1833.sgRNA MSMEG_1833 - gatataatctgggaACACAGGTCCCAGGAGTGAGGTgtttttgtactcga hypothetical protein MSMEG_1833 YP_886203.1
pMSMEG_1834.sgRNA MSMEG_1834 manB gatataatctgggaGAGCATTCCCGAGTCGGCGCCgtttttgtactcga phosphomannomutase YP_886204.1
pMSMEG_1836.sgRNA MSMEG_1836 manA gatataatctgggaGCGTGGAATTCCTCAAGTGCCAgtttttgtactcga mannose6phosphate isomerase YP_886206.1
pMSMEG_1843.sgRNA MSMEG_1843 - gatataatctgggaATGGTGCTCCGCGGCGCCCAGTAgtttttgtactcga Pseudogene
pMSMEG_1873.sgRNA MSMEG_1873 - gatataatctgggaGCGTTCGACGCCACGTACCGGTgtttttgtactcga thymidylate kinase YP_886239.1
pMSMEG_1874.sgRNA MSMEG_1874 mtrA gatataatctgggaGCGGTGTCGAAACCCTCACCACgtttttgtactcga DNAbinding response regulator MtrA YP_886240.1
pMSMEG_1875.sgRNA MSMEG_1875 - gatataatctgggaGCACGCGCGACGGATCGGACCGgtttttgtactcga sensor histidine kinase MtrB YP_886241.1
pMSMEG_1876.sgRNA MSMEG_1876 - gatataatctgggaGTTCGACCGTGCCGATCGCCTGCgtttttgtactcga lipoprotein LpqB YP_886242.1
pMSMEG_1889.sgRNA MSMEG_1889 - gatataatctgggaGTCCTCGATGGTGTCCGCGAGATCgtttttgtactcga ribosome small subunitdependent GTPase RsgA YP_886255.1
pMSMEG_1890.sgRNA MSMEG_1890 aroA gatataatctgggaGCCGCCGGTGACCAGCGCGGCGGgtttttgtactcga 3phosphoshikimate 1carboxyvinyltransferase YP_886256.1
pMSMEG_1918.sgRNA MSMEG_1918 - gatataatctgggaACGCGGCCTTGATCTCGGTGTgtttttgtactcga sensor histidine kinase YP_886284.1
pMSMEG_1919.sgRNA MSMEG_1919 - gatataatctgggaGGCCGGCCCACTGTTTCCCACgtttttgtactcga transcription factor WhiB YP_886285.1
pMSMEG_1926.sgRNA MSMEG_1926 - gatataatctgggaACCCAGCGGGCCAGGACGGCGCGgtttttgtactcga acid phosphatase YP_886292.1
pMSMEG_1937.sgRNA MSMEG_1937 - gatataatctgggaGCCCTGCAGATGCACCGCATCgtttttgtactcga molybdopterin biosynthesislike protein MoeZ YP_886303.1
pMSMEG_1938.sgRNA MSMEG_1938 - gatataatctgggaGCCGCCCTGCAGATGCACCGCATCgtttttgtactcga hypothetical protein MSMEG_1938 YP_886304.1
pMSMEG_1952.sgRNA MSMEG_1952 - gatataatctgggaACCGCCGCGTCGGGGAAACGCCGgtttttgtactcga ATPdependent DNA helicase YP_886318.1
pMSMEG_1954.sgRNA MSMEG_1954 - gatataatctgggaGTCTTCCACTACAACCGCAAATGGCTgtttttgtactcga ABC transporter YP_886320.1
pMSMEG_2083.sgRNA MSMEG_2083 - gatataatctgggaGCCCTCGGCGACCATGCAGTAGGAgtttttgtactcga inositol monophosphatase YP_886444.1
pMSMEG_2085.sgRNA MSMEG_2085 - gatataatctgggaGCCCCAGCGCGGGCCCGACCACTCgtttttgtactcga Pseudogene
pMSMEG_2086.sgRNA MSMEG_2086 prfB gatataatctgggaGTCTGCTTGACGGTCAGGATCCgtttttgtactcga peptide chain release factor 2 YP_886446.1
pMSMEG_2089.sgRNA MSMEG_2089 ftsE gatataatctgggaGTCTGCTCCAGCAGAAGACGGTGTTgtttttgtactcga cell division ATPbinding protein FtsE YP_886449.1
pMSMEG_2291.sgRNA MSMEG_2291 - gatataatctgggaACCTCATGGGGCCCATCCACGTCATgtttttgtactcga short chain dehydrogenase YP_886641.1
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