MSRdb: Mycobacterial Systems Resource


CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_2297.sgRNA MSMEG_2297 - gatataatctgggaGAGTGCCTTGATGCGGTCGGGgtttttgtactcga glutaredoxin YP_886647.1
pMSMEG_2298.sgRNA MSMEG_2298 - gatataatctgggaGTCTCGGAAAACACGCATCGTTTCGTgtttttgtactcga Pseudogene
pMSMEG_2299.sgRNA MSMEG_2299 - gatataatctgggaGGCACCCAGCTGATTGGCGTAgtttttgtactcga ribonucleotidediphosphate reductase subunit alpha YP_886648.1
pMSMEG_2313.sgRNA MSMEG_2313 nrdF2 gatataatctgggaATCTCGGCGGTCGAGCACAGCGTgtttttgtactcga ribonucleotidediphosphate reductase subunit beta YP_886661.1
pMSMEG_2317.sgRNA MSMEG_2317 - gatataatctgggaATCTCCGGCCGGACGTCGTGTTCGGCgtttttgtactcga alcohol dehydrogenase YP_886664.1
pMSMEG_2320.sgRNA MSMEG_2320 - gatataatctgggaGCGCCATGAGGCCGCCGACCAGgtttttgtactcga Pseudogene
pMSMEG_2321.sgRNA MSMEG_2321 serB gatataatctgggaGCCACGTCGGACGGCGCGGCCAgtttttgtactcga phosphoserine phosphatase YP_886667.1
pMSMEG_2326.sgRNA MSMEG_2326 - gatataatctgggaACGATGAGCGGGTGCCCACAACCgtttttgtactcga molybdenum ABC transporter ATPase YP_886672.1
pMSMEG_2327.sgRNA MSMEG_2327 - gatataatctgggaGTCGTTCGCCGACTTCCTGCGCGAgtttttgtactcga nudix hydrolase YP_886673.1
pMSMEG_2328.sgRNA MSMEG_2328 - gatataatctgggaGTTCGGGGATGTCGTCGCCCGCgtttttgtactcga enoylCoA hydratase YP_886674.1
pMSMEG_2329.sgRNA MSMEG_2329 - gatataatctgggaGCCGGACTGGATGAGGTTGAGgtttttgtactcga UbiE/COQ5 family methyltransferase YP_886675.1
pMSMEG_2333.sgRNA MSMEG_2333 - gatataatctgggaAAACTTCCGGCATCATGCCGGgtttttgtactcga hypothetical protein MSMEG_2333 YP_886680.1
pMSMEG_2335.sgRNA MSMEG_2335 - gatataatctgggaACCGCGGCCGATCAGGGTGTTGCgtttttgtactcga hexapeptide transferase YP_886681.1
pMSMEG_2348.sgRNA MSMEG_2348 - gatataatctgggaACCGCGCGCACGCCACCCTTCGGgtttttgtactcga Pseudogene
pMSMEG_2349.sgRNA MSMEG_2349 - gatataatctgggaGCCCGTGGTGTGGTCGTAGGTgtttttgtactcga glycosyl hydrolase family protein YP_886692.1
pMSMEG_2350.sgRNA MSMEG_2350 - gatataatctgggaGCTCGCCGGTGAGGGGTAAAGCCGgtttttgtactcga hypothetical protein MSMEG_2350 YP_886693.1
pMSMEG_2352.sgRNA MSMEG_2352 etfA gatataatctgggaGGTCTTGCCGGTCTGGCCGACgtttttgtactcga electron transfer flavoprotein subunit alpha YP_886695.1
pMSMEG_2357.sgRNA MSMEG_2357 - gatataatctgggaGCAGCGAATCCCCTTCGCAGCCgtttttgtactcga cysteine desulfurase YP_886700.1
pMSMEG_2362.sgRNA MSMEG_2362 ligA gatataatctgggaGCTCCTCGAAGTCGGCGACACGgtttttgtactcga NADdependent DNA ligase LigA YP_886705.1
pMSMEG_2364.sgRNA MSMEG_2364 gatC gatataatctgggaGCCGACGGCCGGTTCGCGGTGCgtttttgtactcga aspartyl/glutamyltRNA amidotransferase subunit C YP_886707.1
pMSMEG_2365.sgRNA MSMEG_2365 gatA gatataatctgggaGGCAGCGACGTGCAGGAACGCgtttttgtactcga aspartyl/glutamyltRNA amidotransferase subunit A YP_886708.1
pMSMEG_2366.sgRNA MSMEG_2366 - gatataatctgggaATCCTCGGCACGGCACGCGTCAACCCgtttttgtactcga 6phosphofructokinase, partial YP_004191801.1
pMSMEG_2367.sgRNA MSMEG_2367 gatB gatataatctgggaGTAGTTCTTCGGCTGGTCCGGgtttttgtactcga aspartyl/glutamyltRNA amidotransferase subunit B YP_886709.1
pMSMEG_2369.sgRNA MSMEG_2369 - gatataatctgggaACCGGGCGCACCCCGTGCCGAgtttttgtactcga LppZ protein YP_886711.1
pMSMEG_2374.sgRNA MSMEG_2374 ilvC gatataatctgggaATCGACAGGTCCGCGTCGTCGTCgtttttgtactcga ketolacid reductoisomerase YP_886716.1
pMSMEG_2378.sgRNA MSMEG_2378 serA gatataatctgggaGCTTGTCGGCTATCAGTACAAgtttttgtactcga D3phosphoglycerate dehydrogenase YP_886720.1
pMSMEG_2379.sgRNA MSMEG_2379 leuB gatataatctgggaGTCGAGCGCGAACCGCAGTTTGAgtttttgtactcga 3isopropylmalate dehydrogenase YP_886721.1
pMSMEG_2387.sgRNA MSMEG_2387 leuC gatataatctgggaACCCGCATCGAACCCGGGACGAgtttttgtactcga isopropylmalate isomerase large subunit YP_886727.1
pMSMEG_2388.sgRNA MSMEG_2388 leuD gatataatctgggaATCATCTTGGGCCACTTCGGCCGgtttttgtactcga isopropylmalate isomerase small subunit YP_886728.1
pMSMEG_2389.sgRNA MSMEG_2389 hup gatataatctgggaACCGCCTTGAACTGCGCACCGGGgtttttgtactcga DNAbinding protein HU YP_886729.1
pMSMEG_2391.sgRNA MSMEG_2391 ppk gatataatctgggaGCATCATGTCGGCGCTGCCGATgtttttgtactcga polyphosphate kinase YP_886731.1
pMSMEG_2395.sgRNA MSMEG_2395 ddl gatataatctgggaATCACCGGACCGTCGTCGGTGAGgtttttgtactcga Dalanylalanine synthetase A YP_886735.1
pMSMEG_2398.sgRNA MSMEG_2398 thiL gatataatctgggaGTGTGGGCTCGCCCAGTCGAGgtttttgtactcga thiamine monophosphate kinase YP_886738.1
pMSMEG_2407.sgRNA MSMEG_2407 - gatataatctgggaGGTGTAGGTGGCGGGGTCGGGgtttttgtactcga morphine 6dehydrogenase YP_886747.1
pMSMEG_2408.sgRNA MSMEG_2408 - gatataatctgggaATGCCTGTGATCACCGGGTCGGgtttttgtactcga 2,5diketoDgluconate reductase A YP_886748.1
pMSMEG_2411.sgRNA MSMEG_2411 - gatataatctgggaGCACGCCCGTCACCAGGACGACgtttttgtactcga integral membrane protein YP_886751.1
pMSMEG_2414.sgRNA MSMEG_2414 coaD gatataatctgggaGTGGCGGTTCTGGTGAACCCCAAgtttttgtactcga phosphopantetheine adenylyltransferase YP_886754.1
pMSMEG_2416.sgRNA MSMEG_2416 - gatataatctgggaGCCGACCGACCGCAGGGTCCCGTgtttttgtactcga hypothetical protein MSMEG_2416 YP_886756.1
pMSMEG_2418.sgRNA MSMEG_2418 rnc gatataatctgggaAGAGGGATTCGACACCGTCGGgtttttgtactcga ribonuclease III YP_886758.1
pMSMEG_2424.sgRNA MSMEG_2424 ftsY gatataatctgggaGGTCGCCGCCGCCGAGCAGGCgtttttgtactcga signal recognition particledocking protein FtsY YP_886764.1
pMSMEG_2430.sgRNA MSMEG_2430 ffh gatataatctgggaATCCGGCTGGCCATGCGGTCGGGgtttttgtactcga signal recognition particle protein YP_886770.1
pMSMEG_2437.sgRNA MSMEG_2437 rimM gatataatctgggaACTCGTCGGGGTCGTCGATCGgtttttgtactcga 16S rRNAprocessing protein RimM YP_886777.1
pMSMEG_2438.sgRNA MSMEG_2438 trmD gatataatctgggaGGCCCGCAGGCGTCGGCACCAgtttttgtactcga tRNA (guanineN(1))methyltransferase YP_886778.1
pMSMEG_2440.sgRNA MSMEG_2440 rplS gatataatctgggaACGTTCAAGGACGTCAACATCgtttttgtactcga 50S ribosomal protein L19 YP_886780.1
pMSMEG_2441.sgRNA MSMEG_2441 lepB gatataatctgggaATGAACGTCAGCATCACGTAGTgtttttgtactcga signal peptidase I YP_886781.1
pMSMEG_2519.sgRNA MSMEG_2519 rpsB gatataatctgggaGCTGCAGGCGCTTGTGCACGGTgtttttgtactcga 30S ribosomal protein S2 YP_886856.1
pMSMEG_2520.sgRNA MSMEG_2520 tsf gatataatctgggaGTCGAGCAGCACGACGTCCTTgtttttgtactcga elongation factor Ts YP_886857.1
pMSMEG_2540.sgRNA MSMEG_2540 pyrH gatataatctgggaGCTGCTGCAGTTGGGCACCGCGgtttttgtactcga uridylate kinase YP_886877.1
pMSMEG_2541.sgRNA MSMEG_2541 frr gatataatctgggaACTCTCTTCGATGCCGAGGAGAAGATgtttttgtactcga ribosome recycling factor YP_886878.1
pMSMEG_2543.sgRNA MSMEG_2543 cdsA gatataatctgggaATCAGCAGCGGGACGACCGGGATgtttttgtactcga phosphatidate cytidylyltransferase YP_886879.1
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