MSRdb: Mycobacterial Systems Resource



CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_2578.sgRNA MSMEG_2578 dxr gatataatctgggaGAACTCCCAGGTCGAGGCCGTgtttttgtactcga 1deoxyDxylulose 5phosphate reductoisomerase YP_886915.1
pMSMEG_2580.sgRNA MSMEG_2580 ispG gatataatctgggaATCGCGGCGAAGATGTACTTGGGgtttttgtactcga 4hydroxy3methylbut2en1yl diphosphate synthase YP_886917.1
pMSMEG_2584.sgRNA MSMEG_2584 - gatataatctgggaGCGCCGGCGATCGTCAACGAGGTgtttttgtactcga penicillinbinding protein YP_886921.1
pMSMEG_2619.sgRNA MSMEG_2619 - gatataatctgggaATGACCCAGCTGCGCCCGGCGgtttttgtactcga efflux protein YP_886956.1
pMSMEG_2625.sgRNA MSMEG_2625 nusA gatataatctgggaGGCGATCTCGGGCACTTCCAGgtttttgtactcga transcription elongation factor NusA YP_886962.1
pMSMEG_2628.sgRNA MSMEG_2628 infB gatataatctgggaGATGCGGTCCTCGTCGACGACgtttttgtactcga translation initiation factor IF2 YP_886965.1
pMSMEG_2648.sgRNA MSMEG_2648 - gatataatctgggaACCTCGGCCGAGGCGACACGGTCCGgtttttgtactcga Sfptype phosphopantetheinyl transferase YP_886985.1
pMSMEG_2653.sgRNA MSMEG_2653 ribF gatataatctgggaACGCCGTCGAACACCCCGATGGgtttttgtactcga bifunctional riboflavin kinase/FMN adenylyltransferase YP_886990.1
pMSMEG_2654.sgRNA MSMEG_2654 rpsO gatataatctgggaGCTGCAGGCCGCGGTGGCTGTGTgtttttgtactcga 30S ribosomal protein S15 YP_886991.1
pMSMEG_2656.sgRNA MSMEG_2656 gpsI gatataatctgggaGCTCCTCGACGTCCACCGTGAGCGGgtttttgtactcga polynucleotide phosphorylase YP_886993.1
pMSMEG_2666.sgRNA MSMEG_2666 - gatataatctgggaGCACCGGGTAGTACACCGTGTCgtttttgtactcga multimeric flavodoxin WrbA YP_887003.1
pMSMEG_2670.sgRNA MSMEG_2670 thyA gatataatctgggaGCGCACGTTGGAGTCGCCACGgtttttgtactcga thymidylate synthase YP_887007.1
pMSMEG_2671.sgRNA MSMEG_2671 folA gatataatctgggaGCGAGGTCCTCGGGCAGACGCCgtttttgtactcga dihydrofolate reductase YP_887008.1
pMSMEG_2683.sgRNA MSMEG_2683 thyX gatataatctgggaACACCGAGGCGTGTTCGAGCACgtttttgtactcga FADdependent thymidylate synthase YP_887018.1
pMSMEG_2684.sgRNA MSMEG_2684 dapA gatataatctgggaACCGACGGCGTCGACGACGGTCCgtttttgtactcga dihydrodipicolinate synthase YP_887019.1
pMSMEG_2690.sgRNA MSMEG_2690 - gatataatctgggaGTAGCCGTCGGAGAAGTCGTCgtttttgtactcga DNA translocase FtsK YP_887024.1
pMSMEG_2691.sgRNA MSMEG_2691 - gatataatctgggaATCTCGACGAAGCCGTGCTTGGCgtttttgtactcga Nacetylglutamate synthase YP_887026.1
pMSMEG_2692.sgRNA MSMEG_2692 pgsA gatataatctgggaGCACGCGGGACCACCGGATCGgtttttgtactcga CDPdiacylglycerolglycerol3phosphate 3phosphatidyltransferase YP_887027.1
pMSMEG_2735.sgRNA MSMEG_2735 dapF gatataatctgggaGCACCTCGACGTTGACGCCGTCgtttttgtactcga diaminopimelate epimerase YP_887068.1
pMSMEG_2740.sgRNA MSMEG_2740 lexA gatataatctgggaGACCGCCTGCGGCGATACGCCgtttttgtactcga LexA repressor YP_887073.1
pMSMEG_2750.sgRNA MSMEG_2750 - gatataatctgggaACGGCAGGCCGATCACGTCGAgtttttgtactcga irondependent repressor IdeR YP_887081.1
pMSMEG_2758.sgRNA MSMEG_2758 - gatataatctgggaGATCCACCACGTGGCGTAGGTgtttttgtactcga RNA polymerase sigma factor YP_887090.1
pMSMEG_2763.sgRNA MSMEG_2763 - gatataatctgggaGCTTTGAGTTCCTCGAGCGAATCCgtttttgtactcga hypothetical protein MSMEG_2763 YP_887094.1
pMSMEG_2765.sgRNA MSMEG_2765 dut gatataatctgggaATTTGGCCACCTCGAGGTCGGCGGCgtttttgtactcga deoxyuridine 5\'triphosphate nucleotidohydrolase YP_887096.1
pMSMEG_2766.sgRNA MSMEG_2766 - gatataatctgggaGTTTGTGATGGCATTCGGTAAGCGgtttttgtactcga hypothetical protein MSMEG_2766 YP_887097.1
pMSMEG_2776.sgRNA MSMEG_2776 dxs gatataatctgggaGGCCTGCGAGATCAACTCGTCgtttttgtactcga 1deoxyDxylulose5phosphate synthase YP_887107.1
pMSMEG_2780.sgRNA MSMEG_2780 hemE gatataatctgggaGGTTGCCCTGCAGCGCGGCGCgtttttgtactcga uroporphyrinogen decarboxylase YP_887111.1
pMSMEG_2781.sgRNA MSMEG_2781 hemG gatataatctgggaGCTGCGGGATCGGCGTCCCGCgtttttgtactcga protoporphyrinogen oxidase YP_887112.1
pMSMEG_2782.sgRNA MSMEG_2782 - gatataatctgggaATCCGGCCCAGCGCGGTGGTGCGgtttttgtactcga hypothetical protein MSMEG_2782 YP_887113.1
pMSMEG_2785.sgRNA MSMEG_2785 - gatataatctgggaGTGTGTTGGTGACCGATTCGGTgtttttgtactcga integral membrane protein YP_887116.1
pMSMEG_2931.sgRNA MSMEG_2931 thrS gatataatctgggaGCTCCTCGACGTCGAAGTCGTAgtttttgtactcga threonyltRNA synthetase YP_887250.1
pMSMEG_2932.sgRNA MSMEG_2932 - gatataatctgggaGCTACATCGCCGAGGCGCCGATgtttttgtactcga HIT family protein hydrolase YP_887251.1
pMSMEG_2933.sgRNA MSMEG_2933 - gatataatctgggaGCTCCGAGCTGATGGCGTTCACgtttttgtactcga phosphatidylinositol synthase YP_887252.1
pMSMEG_2934.sgRNA MSMEG_2934 - gatataatctgggaGGGCATCCGGGTGGCCTCGCCgtttttgtactcga lipid A biosynthesis lauroyl acyltransferase YP_887253.1
pMSMEG_2935.sgRNA MSMEG_2935 - gatataatctgggaGGTGGCGTCGTCGACCTGGCCgtttttgtactcga phosphatidylinositol alphamannosyltransferase YP_887254.1
pMSMEG_2936.sgRNA MSMEG_2936 - gatataatctgggaGGCGAACCGTGACGAGACGGCCgtttttgtactcga hydrolase, nudix family protein YP_887255.1
pMSMEG_2940.sgRNA MSMEG_2940 - gatataatctgggaGCTGTACGACGCCATCCAGAAGGCgtttttgtactcga hypothetical protein MSMEG_2940 YP_887259.1
pMSMEG_2943.sgRNA MSMEG_2943 ruvC gatataatctgggaGCACGCCCGCCGACACGCCGCTgtttttgtactcga Holliday junction resolvase YP_887262.1
pMSMEG_2945.sgRNA MSMEG_2945 ruvB gatataatctgggaGCGCGGCGCTGTCCATGTTGGGgtttttgtactcga Holliday junction DNA helicase RuvB YP_887264.1
pMSMEG_2961.sgRNA MSMEG_2961 secD gatataatctgggaACGAGGAACACGACGACGAGGTgtttttgtactcga preprotein translocase subunit SecD YP_887280.1
pMSMEG_2962.sgRNA MSMEG_2962 secF gatataatctgggaACACGATGACCGTGTCGTACAGgtttttgtactcga preprotein translocase subunit SecF YP_887281.1
pMSMEG_2963.sgRNA MSMEG_2963 - gatataatctgggaGTTCGGTGGACTGCGAACCCGGgtttttgtactcga extracellular solutebinding protein YP_887282.1
pMSMEG_2965.sgRNA MSMEG_2965 relA gatataatctgggaGCCCAAGCACTACTGGTCCATCTAgtttttgtactcga GTP pyrophosphokinase YP_887284.1
pMSMEG_2974.sgRNA MSMEG_2974 - gatataatctgggaGTCTTGCCTGCGCCGTTGGGGCCGATgtttttgtactcga cyclophilin type peptidylprolyl cistrans isomerase YP_887293.1
pMSMEG_2975.sgRNA MSMEG_2975 - gatataatctgggaAGTTGCACGCCAGCATCCCGGgtttttgtactcga metallobetalactamase YP_887294.1
pMSMEG_2976.sgRNA MSMEG_2976 hisS gatataatctgggaGCCCCGAGTAGCAGAGCTTGAgtttttgtactcga histidyltRNA synthetase YP_887295.1
pMSMEG_3003.sgRNA MSMEG_3003 aspS gatataatctgggaGACGACGCCGGTCACCGCGACgtttttgtactcga aspartyltRNA synthetase YP_887321.1
pMSMEG_3025.sgRNA MSMEG_3025 alaS gatataatctgggaGCTTGCCGTCGACGAAGATGCgtttttgtactcga alanyltRNA synthetase YP_887342.1
pMSMEG_3026.sgRNA MSMEG_3026 - gatataatctgggaGCCGCTGATCGAGCCAGCCTTgtttttgtactcga Holliday junction resolvaselike protein YP_887343.1
pMSMEG_3028.sgRNA MSMEG_3028 aroE gatataatctgggaGAACTTGCCCGGCATGGTCACgtttttgtactcga shikimate 5dehydrogenase YP_887345.1
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