MSRdb: Mycobacterial Systems Resource



CRISPRi Clones

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CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_3030.sgRNA MSMEG_3030 aroC gatataatctgggaGCTTGAGCTGTGCCCCGATCTCCgtttttgtactcga chorismate synthase YP_887347.1
pMSMEG_3031.sgRNA MSMEG_3031 aroK gatataatctgggaGTCTCCTCGATCACCGCGTCGGTGTgtttttgtactcga shikimate kinase YP_887348.1
pMSMEG_3033.sgRNA MSMEG_3033 aroB gatataatctgggaGCCTCGGGATCGGCCTCGATGAGGTgtttttgtactcga 3dehydroquinate synthase YP_887349.1
pMSMEG_3035.sgRNA MSMEG_3035 efp gatataatctgggaATCTGCACCGGCATGCTCTCCAGgtttttgtactcga elongation factor P YP_887352.1
pMSMEG_3036.sgRNA MSMEG_3036 nusB gatataatctgggaGCCGCCCGGATCTGCGGCGTCAgtttttgtactcga transcription antitermination protein NusB YP_887353.1
pMSMEG_3043.sgRNA MSMEG_3043 pyrB gatataatctgggaACCGAGTACTCACGCGCGGACGGgtttttgtactcga aspartate carbamoyltransferase YP_887359.1
pMSMEG_3044.sgRNA MSMEG_3044 pyrC gatataatctgggaATCCCTTGTGCCTTGGCCCATTgtttttgtactcga dihydroorotase YP_887360.1
pMSMEG_3046.sgRNA MSMEG_3046 carA gatataatctgggaGTCTCCTGGTAGCCCGACATGCCGGTgtttttgtactcga carbamoyl phosphate synthase small subunit YP_887362.1
pMSMEG_3048.sgRNA MSMEG_3048 pyrF gatataatctgggaGGCCGTTGGCCACCGCGGTGTgtttttgtactcga orotidine 5\'phosphate decarboxylase YP_887364.1
pMSMEG_3050.sgRNA MSMEG_3050 - gatataatctgggaACGAACAGCGCGCGGCAGCGTTgtttttgtactcga integration host factor YP_887366.1
pMSMEG_3051.sgRNA MSMEG_3051 gmk gatataatctgggaACGGACACGGAAAAGTACAGGTgtttttgtactcga guanylate kinase YP_887367.1
pMSMEG_3053.sgRNA MSMEG_3053 rpoZ gatataatctgggaATCGGCGTGCGGGGTGCTCACgtttttgtactcga DNAdirected RNA polymerase subunit omega YP_887369.1
pMSMEG_3054.sgRNA MSMEG_3054 coaBC gatataatctgggaGCCTTGACCCCGGCCATGTCGTgtttttgtactcga bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase YP_887370.1
pMSMEG_3055.sgRNA MSMEG_3055 metK gatataatctgggaGACCTTTGACGGATCCTTGCCgtttttgtactcga Sadenosylmethionine synthetase YP_887371.1
pMSMEG_3061.sgRNA MSMEG_3061 priA gatataatctgggaGCCCAGCAGCGCCCACGCGTCGAgtttttgtactcga primosome assembly protein PriA YP_887377.1
pMSMEG_3066.sgRNA MSMEG_3066 rpe gatataatctgggaGCCAGCCGCGCGAAATCCGCGGgtttttgtactcga ribulosephosphate 3epimerase YP_887382.1
pMSMEG_3067.sgRNA MSMEG_3067 ribD gatataatctgggaACCGGCCAGCGTCGGGCCGCCCTgtttttgtactcga riboflavin biosynthesis protein RibD YP_887383.1
pMSMEG_3071.sgRNA MSMEG_3071 ribE gatataatctgggaACCGCCGACCTGATCGCGTTCGGgtttttgtactcga riboflavin synthase subunit alpha YP_887387.1
pMSMEG_3072.sgRNA MSMEG_3072 - gatataatctgggaATCGACCGGATCCCGAGGTCCAgtttttgtactcga bifunctional 3,4dihydroxy2butanone 4phosphate synthase/GTP cyclohydrolase II protein YP_887388.1
pMSMEG_3081.sgRNA MSMEG_3081 - gatataatctgggaATGTGCAGGCCGCCGGCAAAACgtttttgtactcga hypothetical protein MSMEG_3081 YP_887397.1
pMSMEG_3084.sgRNA MSMEG_3084 gap gatataatctgggaGCCTTCTGCGTCGCCAGGGCCCGgtttttgtactcga glyceraldehyde3phosphate dehydrogenase YP_887400.1
pMSMEG_3085.sgRNA MSMEG_3085 pgk gatataatctgggaGCCACCACCGGTGGAGATGTGgtttttgtactcga phosphoglycerate kinase YP_887401.1
pMSMEG_3086.sgRNA MSMEG_3086 tpiA gatataatctgggaACGCCCGCCGCGATCTCGGACgtttttgtactcga triosephosphate isomerase YP_887402.1
pMSMEG_3103.sgRNA MSMEG_3103 tkt gatataatctgggaGCCGCATGTAATCCGAGAACTGgtttttgtactcga transketolase YP_887418.1
pMSMEG_3105.sgRNA MSMEG_3105 cyoE gatataatctgggaGTTGGTGGACAACCACAGCCAgtttttgtactcga protoheme IX farnesyltransferase YP_887420.1
pMSMEG_3120.sgRNA MSMEG_3120 - gatataatctgggaACGAACCCCAGCGCGAGCAGCGgtttttgtactcga hypothetical protein MSMEG_3120 YP_887435.1
pMSMEG_3121.sgRNA MSMEG_3121 - gatataatctgggaGCCATCACAGCAGCGTAACCGGCgtttttgtactcga DNAbinding protein YP_887436.1
pMSMEG_3122.sgRNA MSMEG_3122 sufB gatataatctgggaACGCAGCGCGGAGTCGGTGTCgtttttgtactcga FeS assembly protein SufB YP_887437.1
pMSMEG_3123.sgRNA MSMEG_3123 sufD gatataatctgggaGGGGATGCCGCGGGCCCGCAGgtttttgtactcga FeS assembly protein SufD YP_887438.1
pMSMEG_3124.sgRNA MSMEG_3124 sufC gatataatctgggaGTGGCGCTTCTTCTCACCGCCgtttttgtactcga FeS assembly ATPase SufC YP_887439.1
pMSMEG_3125.sgRNA MSMEG_3125 - gatataatctgggaGCGCATCACCCGGCTCAGGATgtttttgtactcga cysteine desulfurase YP_887440.1
pMSMEG_3126.sgRNA MSMEG_3126 - gatataatctgggaGATGCGGATGCTTGTAGTGGTgtttttgtactcga SUF system FeS assembly protein, NifU family protein YP_887441.1
pMSMEG_3143.sgRNA MSMEG_3143 acnA gatataatctgggaGCCTCGATACCGCCGACGCCCCAGCgtttttgtactcga aconitate hydratase 1 YP_887458.1
pMSMEG_3145.sgRNA MSMEG_3145 - gatataatctgggaGCCCCCGCCGTACAGCGCCGTTGgtttttgtactcga secreted cell wallassociated hydrolase YP_887460.1
pMSMEG_3147.sgRNA MSMEG_3147 moxR gatataatctgggaGCGGTTGATCTCGTCGGCGAGgtttttgtactcga ATPase, MoxR family protein YP_887462.1
pMSMEG_3148.sgRNA MSMEG_3148 - gatataatctgggaGCGCATCCAGTTGATGGGCCCgtttttgtactcga hypothetical protein MSMEG_3148 YP_887463.1
pMSMEG_3149.sgRNA MSMEG_3149 - gatataatctgggaAGCCGGGCAGGCGGCGGTTGAgtttttgtactcga hypothetical protein MSMEG_3149 YP_887464.1
pMSMEG_3150.sgRNA MSMEG_3150 fabG gatataatctgggaGTAGGAGGCGTCCTCCGAGGCgtttttgtactcga 3oxoacylACP reductase YP_887465.1
pMSMEG_3151.sgRNA MSMEG_3151 - gatataatctgggaACACATCCTCGTACGGCGCGTCgtttttgtactcga enoylACP reductase YP_887466.1
pMSMEG_3152.sgRNA MSMEG_3152 hemH gatataatctgggaAGTTGCGGTTGCCGAAGTAGAgtttttgtactcga ferrochelatase YP_887467.1
pMSMEG_3159.sgRNA MSMEG_3159 - gatataatctgggaGTGTCCGAGTTCGAGCCGAAGAGgtttttgtactcga methylmalonylCoA mutase YP_887474.1
pMSMEG_3169.sgRNA MSMEG_3169 ileS gatataatctgggaGGTGGCCAGCACGTGCAGCGTgtttttgtactcga isoleucyltRNA synthetase YP_887484.1
pMSMEG_3178.sgRNA MSMEG_3178 dnaE gatataatctgggaGCCGGCCTTGGTCGCCGAGTTgtttttgtactcga DNA polymerase III subunit alpha YP_887493.1
pMSMEG_3183.sgRNA MSMEG_3183 ilvA gatataatctgggaGGGTTCCTGCGGGAAGTCCACgtttttgtactcga threonine dehydratase YP_887498.1
pMSMEG_3188.sgRNA MSMEG_3188 bioA gatataatctgggaACCCGGCCTACAGCGCGGCGTTgtttttgtactcga adenosylmethionine8amino7oxononanoate aminotransferase YP_887502.1
pMSMEG_3194.sgRNA MSMEG_3194 bioB gatataatctgggaGCGGTGTCGGTGCGCGCGGTGCgtttttgtactcga biotin synthase YP_887508.1
pMSMEG_3195.sgRNA MSMEG_3195 - gatataatctgggaATCACCCTGGGCGACCTCGGCGCgtttttgtactcga hypothetical protein MSMEG_3195 YP_887509.1
pMSMEG_3199.sgRNA MSMEG_3199 nadA gatataatctgggaGTCTCGGCCATGAAGTGCACGCCgtttttgtactcga quinolinate synthetase YP_887513.1
pMSMEG_3200.sgRNA MSMEG_3200 nadB gatataatctgggaACCTCCTGTGTGGGGTCGGTGTCgtttttgtactcga Laspartate oxidase YP_887514.1
pMSMEG_3201.sgRNA MSMEG_3201 nadC gatataatctgggaGGCCATCTGCGTCTGCCACACgtttttgtactcga nicotinatenucleotide pyrophosphorylase YP_887515.1
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