MSRdb: Mycobacterial Systems Resource



CRISPRi Clones

Page of 14
CRISPRi Plasmid Gene Locus sgRNA Sequence Protein Name Protein Product
pMSMEG_3205.sgRNA MSMEG_3205 hisD gatataatctgggaACGCCCGCGTCGATGTCGTCGAgtttttgtactcga histidinol dehydrogenase YP_887519.1
pMSMEG_3206.sgRNA MSMEG_3206 hisC gatataatctgggaGGCCAGTTCGGCGCCGGCGGCgtttttgtactcga histidinolphosphate aminotransferase YP_887520.1
pMSMEG_3207.sgRNA MSMEG_3207 hisB gatataatctgggaGCCCAGCGAGGTGAGCATGTGGTCgtttttgtactcga imidazoleglycerolphosphate dehydratase YP_887521.1
pMSMEG_3208.sgRNA MSMEG_3208 hisH gatataatctgggaGCGCGGCGTAGGAATGCACGAAgtttttgtactcga imidazole glycerol phosphate synthase subunit HisH YP_887522.1
pMSMEG_3209.sgRNA MSMEG_3209 - gatataatctgggaGTCTCCCAGCCGCGGCCACGCAGgtttttgtactcga phosphoribosyl isomerase A YP_887523.1
pMSMEG_3211.sgRNA MSMEG_3211 hisF gatataatctgggaGGCGACGGTCAGTTCGCGGAAgtttttgtactcga imidazole glycerol phosphate synthase subunit HisF YP_887525.1
pMSMEG_3212.sgRNA MSMEG_3212 hisI gatataatctgggaACCCACTGCTCACCGCGTGAACGgtttttgtactcga phosphoribosylAMP cyclohydrolase YP_887526.1
pMSMEG_3217.sgRNA MSMEG_3217 trpE gatataatctgggaGCCGGCGCACCATGTCATAGGCgtttttgtactcga anthranilate synthase component I YP_887531.1
pMSMEG_3219.sgRNA MSMEG_3219 trpC gatataatctgggaATTCCGCGACACGGATGACGTTGCgtttttgtactcga indole3glycerolphosphate synthase YP_887533.1
pMSMEG_3220.sgRNA MSMEG_3220 trpB gatataatctgggaGTGGTTCAAGTCTTCCCGCTTgtttttgtactcga tryptophan synthase subunit beta YP_887534.1
pMSMEG_3221.sgRNA MSMEG_3221 trpA gatataatctgggaATGCGGTCGAGGTCGTTCGCCgtttttgtactcga tryptophan synthase subunit alpha YP_887535.1
pMSMEG_3222.sgRNA MSMEG_3222 - gatataatctgggaGAACTTGCGGTCGACCCAGATgtttttgtactcga prolipoprotein diacylglyceryl transferase YP_887536.1
pMSMEG_3225.sgRNA MSMEG_3225 - gatataatctgggaGCTCCCCCGGTCTCCGGAGCCGTCACgtttttgtactcga ferredoxindependent glutamate synthase 1 YP_887539.1
pMSMEG_3226.sgRNA MSMEG_3226 - gatataatctgggaGCACAACCGTCCGGTGAACTCgtttttgtactcga glutamate synthase NADH/NADPH small subunit YP_887540.1
pMSMEG_3308.sgRNA MSMEG_3308 - gatataatctgggaACGCGACGTCCCTGGCGTCCCgtttttgtactcga universal stress protein family protein YP_887617.1
pMSMEG_3517.sgRNA MSMEG_3517 - gatataatctgggaGCAGCTCGACCGAGTTCTGGACgtttttgtactcga HADsuperfamily hydrolase YP_887820.1
pMSMEG_3528.sgRNA MSMEG_3528 - gatataatctgggaAAGATCCATGCCGGCATGACGgtttttgtactcga ErfK/YbiS/YcfS/YnhG family protein YP_887832.1
pMSMEG_3618.sgRNA MSMEG_3618 - gatataatctgggaACCCGCGTACCGGGGAACGGCATgtttttgtactcga hypothetical protein MSMEG_3618 YP_887921.1
pMSMEG_3621.sgRNA MSMEG_3621 ndh gatataatctgggaGCAAGAAGGCGCGGGCCCGTCTgtttttgtactcga NADH dehydrogenase YP_887924.1
pMSMEG_3634.sgRNA MSMEG_3634 - gatataatctgggaGCCGTATCGACGCCGAGCCGGGgtttttgtactcga inosine 5monophosphate dehydrogenase YP_887936.1
pMSMEG_3640.sgRNA MSMEG_3640 glcB gatataatctgggaGACCACCTTGTCGACACCCGAgtttttgtactcga malate synthase G YP_887943.1
pMSMEG_3642.sgRNA MSMEG_3642 gcvP gatataatctgggaGGGGAGCTGCACGACGACGCCgtttttgtactcga glycine dehydrogenase YP_887945.1
pMSMEG_3644.sgRNA MSMEG_3644 - gatataatctgggaGCCGCCATCGGCGCGCTCGCTgtttttgtactcga MerR family transcriptional regulator YP_887947.1
pMSMEG_3646.sgRNA MSMEG_3646 - gatataatctgggaGATCTTGGAGATGGTCACGTCgtttttgtactcga MerR family transcriptional regulator YP_887949.1
pMSMEG_3647.sgRNA MSMEG_3647 - gatataatctgggaGTCCAGTTCGTTCAGGAAGTCGGCgtttttgtactcga forkheadassociated protein YP_887950.1
pMSMEG_3648.sgRNA MSMEG_3648 gcvH gatataatctgggaACCGCGCGGTAGCCCTCCGCGTgtttttgtactcga glycine cleavage system protein H YP_887951.1
pMSMEG_3652.sgRNA MSMEG_3652 - gatataatctgggaGCGGCGAGCACGGCGTCACGGCgtttttgtactcga CDPdiacylglycerolglycerol3phosphate 3phosphatidyltransferase YP_887956.1
pMSMEG_3654.sgRNA MSMEG_3654 azi gatataatctgggaATCCACCTGCGGGCACTGGGCAGgtttttgtactcga preprotein translocase subunit SecA YP_887957.1
pMSMEG_3738.sgRNA MSMEG_3738 engA gatataatctgggaGGTGTCAACCTTGTTGGCCGCgtttttgtactcga GTPbinding protein EngA YP_888037.1
pMSMEG_3739.sgRNA MSMEG_3739 cmk gatataatctgggaGCGTTCCTCGGCCGACGCGGTgtttttgtactcga cytidylate kinase YP_888038.1
pMSMEG_3740.sgRNA MSMEG_3740 rluB gatataatctgggaGGTACGCACCAACTCCTGCACgtttttgtactcga ribosomal large subunit pseudouridine synthase B YP_888039.1
pMSMEG_3742.sgRNA MSMEG_3742 scpA gatataatctgggaACACCTGGCAGCCGCCGATGCGgtttttgtactcga segregation and condensation protein A YP_888041.1
pMSMEG_3743.sgRNA MSMEG_3743 soj gatataatctgggaGAGCAGCGCCAGGCCGCGTAGgtttttgtactcga SpoOJ regulator protein YP_888042.1
pMSMEG_3744.sgRNA MSMEG_3744 xerD gatataatctgggaGTCGTGGTGACCGACGCGTGGCgtttttgtactcga sitespecific tyrosine recombinase XerD YP_888043.1
pMSMEG_3746.sgRNA MSMEG_3746 pyrG gatataatctgggaATGGCCGTCGGGCGAGGTTCCgtttttgtactcga CTP synthetase YP_888045.1
pMSMEG_3747.sgRNA MSMEG_3747 - gatataatctgggaGCGCGCCGCGGGTGATCGGTAATgtttttgtactcga Pseudogene
pMSMEG_3748.sgRNA MSMEG_3748 - gatataatctgggaAGGTTCGGGTAGCGCCCCGAGgtttttgtactcga thiamin pyrophosphokinase catalytic subunit YP_888046.1
pMSMEG_3749.sgRNA MSMEG_3749 - gatataatctgggaATCGTCTGACGGCGTACTCCCGgtttttgtactcga Pseudogene
pMSMEG_3750.sgRNA MSMEG_3750 ppnK gatataatctgggaATGGCCTCGGCCTCGGCTTCGGgtttttgtactcga inorganic polyphosphate/ATPNAD kinase YP_888047.1
pMSMEG_3758.sgRNA MSMEG_3758 tyrS gatataatctgggaGCTGTTCGACAGCCCGGTCGCCAgtttttgtactcga tyrosyltRNA synthetase YP_888052.1
pMSMEG_3759.sgRNA MSMEG_3759 - gatataatctgggaGTCGCCGGCGTGCGGCTGGTGCGgtttttgtactcga 3methyladenine DNA glycosylase YP_888053.1
pMSMEG_3769.sgRNA MSMEG_3769 argH gatataatctgggaGCGCCAGCAGATGGTGCGCCAgtttttgtactcga argininosuccinate lyase YP_888063.1
pMSMEG_3770.sgRNA MSMEG_3770 argG gatataatctgggaAGAGCGCGTTGGACTGGATGGgtttttgtactcga argininosuccinate synthase YP_888064.1
pMSMEG_3772.sgRNA MSMEG_3772 argF gatataatctgggaGCGGACCCTCCAGCGGTCGCCGgtttttgtactcga ornithine carbamoyltransferase YP_888066.1
pMSMEG_3773.sgRNA MSMEG_3773 argD gatataatctgggaGGCCTCGGTGCCGGAGTTGCAgtttttgtactcga acetylornithine aminotransferase YP_888067.1
pMSMEG_3774.sgRNA MSMEG_3774 argB gatataatctgggaGAGCACCTCGGGCGTGGTGACgtttttgtactcga acetylglutamate kinase YP_888068.1
pMSMEG_3775.sgRNA MSMEG_3775 argJ gatataatctgggaACCAGCGCCAGGTCCAGCGCTgtttttgtactcga bifunctional ornithine acetyltransferase/Nacetylglutamate synthase YP_888069.1
pMSMEG_3776.sgRNA MSMEG_3776 argC gatataatctgggaGCCGGATGGCCCAGCAGCAGCCgtttttgtactcga Nacetylgammaglutamylphosphate reductase YP_888070.1
pMSMEG_3777.sgRNA MSMEG_3777 pheT gatataatctgggaGCGCAACGATCAAGGGCGGCAgtttttgtactcga phenylalanyltRNA synthetase subunit beta YP_888071.1
pMSMEG_3778.sgRNA MSMEG_3778 pheS gatataatctgggaGGCCGACGGCTCGGTGAACGGgtttttgtactcga phenylalanyltRNA synthetase subunit alpha YP_888072.1
Prev
Next